ATP5F1D Protein (AA 23-168) (His tag)
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- 抗原 See all ATP5F1D 蛋白
- ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-168
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ATP5F1D protein is labelled with His tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- 序列
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AEAAAAPAPA AGPGQMSFTF ASPTQVFFDS ANVKQVDVPT LTGAFGILAS HVPTLQVLRP GLVVVHTEDG TTTKYFVSSG SVTVNADSSV QLLAEEAVTL DMLDLGAARA NLEKAQSELS GAADEAARAE IQIRIEANEA LVKALE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Atp5d Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ATP5F1D 蛋白
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ATP synthase subunit delta, mitochondrial (ATP5F1D) (AA 23-168) protein (His tag)
ATP5F1D 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ATP synthase subunit delta, mitochondrial (ATP5F1D) (AA 43-161), (partial) protein (GST tag)ATP5F1D 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
ATP synthase subunit delta, mitochondrial (ATP5F1D) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ATP5F1D 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ATP synthase subunit delta, mitochondrial (ATP5F1D) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ATP5F1D 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))
- 别名
- Atp5d (ATP5F1D 产品)
- 别名
- fk58f09 Protein, zgc:73303 Protein, wu:fk58f09 Protein, MGC85306 Protein, GB17255 Protein, DDBDRAFT_0216607 Protein, DDBDRAFT_0238330 Protein, DDB_0216607 Protein, DDB_0238330 Protein, 0610008F14Rik Protein, 1500000I11Rik Protein, AA960090 Protein, AI876556 Protein, AU020773 Protein, C85518 Protein, ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit Protein, ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit L homeolog Protein, ATP synthase F1 delta Protein, atp5d Protein, atp5d.L Protein, ATP5D Protein, LOC552682 Protein, atp5D Protein, Atp5d Protein
- 背景
- Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
- 分子量
- 16.0 kDa Including tag.
- UniProt
- Q9D3D9
- 途径
- Proton Transport, Ribonucleoside Biosynthetic Process
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