KARS Protein (AA 2-595) (His tag)
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- 抗原 See all KARS 蛋白
- KARS (Lysyl-tRNA Synthetase (KARS))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-595
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This KARS protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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ATLQESEVKV DGEQKLSKNE LKRRLKAEKK LAEKEAKQKE LSEKQLNQTA SAPNHTADNG VGAEEETLDP NQYYKIRSQA VQQLKVTGED PYPHKFHVDI SLTQFIQEYS HLQPGDHLTD VTLKVAGRIH AKRASGGKLI FYDLRGEGVK LQVMANSRNY KSEEEFVHIN NKLRRGDIIG VEGNPGKTKK GELSIIPQEI TLLSPCLHML PHLHFGLKDK ETRYRQRYLD LILNDFVRQK FIVRSKIITY IRSFLDELGF LEIETPMMNI IPGGAVAKPF ITYHNELDMN LYMRIAPELY HKMLVVGGID RVYEIGRQFR NEGIDLTHNP EFTTCEFYMA YADYHDLMEI TEKMLSGMVK SITGSYKITY HPDGPEGQAY EVDFTPPFRR ISMVEELEKA LGVKLPETSL FETEETRKIL DDICVAKAVE CPPPRTTARL LDKLVGEFLE VTCISPTFIC DHPQIMSPLA KWHRSKEGLT ERFELFVMKK EICNAYTELN DPVRQRQLFE EQAKAKAAGD DEAMFIDENF CTALEYGLPP TAGWGMGIDR LTMFLTDSNN IKEVLLFPAM KPEDKKETAA TTETPESTEA SPSV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Kars Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product KARS 蛋白
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Lysyl-tRNA Synthetase (KARS) (AA 1-597) protein (GST tag)
KARS 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Lysyl-tRNA Synthetase (KARS) (AA 63-597) protein (His tag)KARS 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Lysyl-tRNA Synthetase (KARS) (AA 2-597) protein (His tag)Crystallography grade KARS 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Lysyl-tRNA Synthetase (KARS) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)KARS 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- KARS (Lysyl-tRNA Synthetase (KARS))
- 别名
- Kars (KARS 产品)
- 别名
- CG12141 Protein, Dmel\\CG12141 Protein, KRS Protein, LysRS Protein, cb530 Protein, wu:fa16h02 Protein, zgc:92483 Protein, kars Protein, MGC53345 Protein, kars2 Protein, krs Protein, krs-1 Protein, KARS Protein, Tb06.28P18.190 Protein, CMTRIB Protein, DFNB89 Protein, KARS1 Protein, KARS2 Protein, AA589550 Protein, AL024334 Protein, AL033315 Protein, AL033367 Protein, D8Ertd698e Protein, D8Wsu108e Protein, mKIAA0070 Protein, Lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase S homeolog Protein, lysyl-tRNA synthetase L homeolog Protein, LysRS Protein, kars Protein, kars.S Protein, kars.L Protein, Bm1_16500 Protein, lysS Protein, Taci_1118 Protein, Tpau_1737 Protein, Arch_0261 Protein, Trad_2960 Protein, Spirs_3699 Protein, KARS Protein, LOC100280510 Protein, Tb927.6.1510 Protein, Kars Protein
- 背景
- Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
- 分子量
- 68.7 kDa Including tag.
- UniProt
- Q99MN1
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