DPP3 Protein (AA 2-738) (His tag)
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- 抗原 See all DPP3 蛋白
- DPP3 (Dipeptidyl-Peptidase 3 (DPP3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-738
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This DPP3 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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ADTQYILPND IGVSSLDCRE AFRLLSPTER LYAHHLSRAA WYGGLAVLLQ TSPEAPYIYA LLSRLFRAQD PDQLRQHALA EGLTEEEYQA FLVYAAGVYS NMGNYKSFGD TKFVPNLPKD KLGRVILGSK AAQQRPEEVR DLWQTCGDLM FSLEPRLRHL GLGKEGVTTY FSGDCTMEDA KLAQDFLDSQ NLSAYNTRLF KVVGQEGKSH YEVRLASVLN TDPALDSELT SKLKRYEFQG NHFQVTRGDY APILQKVVEH LEKAKAYAAN SHQEQMLAQY VESFTQGSIE AHKRGSRFWI QDKGPIVESY IGFIESYRDP FGSRGEFEGF VAMVNKAMSA KFERLVASAE QLLKELPWPL AFEKDKFLTP DFTSLDVLTF AGSGIPAGIN IPNYDDLRQT EGFKNVSLGN VLAVAYAAKR EKLTFLEEED KDLYIRWKGP SFDVQVGLHE LLGHGSGKLF VQDEKGAFNF DKETVINPET GEQIQSWYRS GETWDSKFST IASSYEECRA ESVGLYLCLN PQVLEIFGFE GADAEDVIYV NWLNMVRAGL LALEFYTPEA ANWRQAHMQA RFVILRVLLE AGEGLVTVTP TTGSDGRPDA RVRLDRSKIR SVGRPALERF LRRLQVLKST GDVVAGRALY EGYAAVTDAP PECFLTLRDT VLLRKESRKL IVQPNTRLEG SEVQLVEYEA SAAGLIRSFC ERFPEDGPEL EEVLIQLAAA DARFWRNQAQ EAPPGQA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Dpp3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product DPP3 蛋白
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Dipeptidyl-Peptidase 3 (DPP3) (AA 593-737) protein (His-IF2DI Tag)
DPP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Dipeptidyl-Peptidase 3 (DPP3) (AA 1-737) protein (GST tag)DPP3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dipeptidyl-Peptidase 3 (DPP3) (AA 424-504) protein (GST tag)DPP3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dipeptidyl-Peptidase 3 (DPP3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DPP3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Dipeptidyl-Peptidase 3 (DPP3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DPP3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- DPP3 (Dipeptidyl-Peptidase 3 (DPP3))
- 别名
- Dpp3 (DPP3 产品)
- 别名
- DPPIII Protein, dppiii Protein, MGC68828 Protein, dpp3 Protein, MGC79125 Protein, DKFZp469J1915 Protein, 4930533O14Rik Protein, C86324 Protein, CG7415 Protein, DPP Protein, DPP III Protein, Dmel\\CG7415 Protein, wu:fb64b04 Protein, zgc:86791 Protein, dipeptidyl peptidase 3 Protein, dipeptidylpeptidase 3 Protein, dipeptidyl-peptidase 3 S homeolog Protein, dipeptidyl-peptidase 3 L homeolog Protein, dipeptidyl-peptidase 3 Protein, Dipeptidyl aminopeptidase III Protein, DPP3 Protein, Dpp3 Protein, dpp3.S Protein, dpp3.L Protein, CpipJ_CPIJ005356 Protein, PTRG_00518 Protein, MCYG_01338 Protein, MGYG_01882 Protein, dpp3 Protein, DppIII Protein
- 背景
- Cleaves and degrades of the opioid peptide enkephalin. Also cleaves Arg-Arg-beta-naphthylamide (By similarity). {ECO:0000250}.
- 分子量
- 83.7 kDa Including tag.
- UniProt
- Q99KK7
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