EpCAM Protein (AA 24-266) (His tag)
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- 抗原 See all EpCAM (EPCAM) 蛋白
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-266
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This EpCAM protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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QRDCVCDNYK LATSCSLNEY GECQCTSYGT QNTVICSKLA SKCLAMKAEM THSKSGRRIK PEGAIQNNDG LYDPDCDEQG LFKAKQCNGT ATCWCVNTAG VRRTDKDTEI TCSERVRTYW IIIELKHKER ESPYDHQSLQ TALQEAFTSR YKLNQKFIKN IMYENNVITI DLMQNSSQKT QDDVDIADVA YYFEKDVKGE SLFHSSKSMD LRVNGEPLDL DPGQTLIYYV DEKAPEFSMQ GLT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Epcam Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EPCAM 蛋白
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Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (His-Avi Tag,Biotin)
EPCAM 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (Fc Tag)EPCAM 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (His tag)EPCAM 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (His tag)EPCAM 宿主: 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (His-DYKDDDDK-Strep II Tag)EPCAM 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Epithelial Cell Adhesion Molecule (EPCAM) (Active) protein (Fc Tag)EPCAM 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Epithelial Cell Adhesion Molecule (EPCAM) protein (His tag)EPCAM 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by reducing SDS-PAGE.
Epithelial Cell Adhesion Molecule (EPCAM) (AA 118-314) protein (His tag)EPCAM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Epithelial Cell Adhesion Molecule (EPCAM) (AA 1-314) protein (GST tag)EPCAM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Epithelial Cell Adhesion Molecule (EPCAM) protein (His tag)EPCAM 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 别名
- Epcam (EPCAM 产品)
- 别名
- DIAR5 Protein, EGP-2 Protein, EGP314 Protein, EGP40 Protein, ESA Protein, HNPCC8 Protein, KS1/4 Protein, KSA Protein, M4S1 Protein, MIC18 Protein, MK-1 Protein, TACSTD1 Protein, TROP1 Protein, ECS-1 Protein, ECS1 Protein, ESA1 Protein, M17S1 Protein, EGP Protein, Ep-CAM Protein, sb:cb6 Protein, tacstd Protein, wu:fj17g02 Protein, zgc:110304 Protein, zgc:77119 Protein, tacstd1 Protein, MGC80540 Protein, EPCAM Protein, egp Protein, ksa Protein, m4s1 Protein, mk-1 Protein, cd326 Protein, egp40 Protein, mic18 Protein, trop1 Protein, ep-cam Protein, hegp-2 Protein, co17-1a Protein, ga733-2 Protein, CD326 Protein, Egp314 Protein, EpCAM1 Protein, GA733-2 Protein, Ly74 Protein, Tacsd1 Protein, Tacstd1 Protein, gp40 Protein, epithelial cell adhesion molecule Protein, flotillin 2 Protein, epithelial cell adhesion molecule S homeolog Protein, EPCAM Protein, FLOT2 Protein, epcam Protein, epcam.S Protein, Epcam Protein
- 背景
- May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity). {ECO:0000250}.
- 分子量
- 28.6 kDa Including tag.
- UniProt
- Q99JW5
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