PSIP1 Protein (AA 1-528) (Strep Tag)
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- 抗原 See all PSIP1 蛋白
- PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-528
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This PSIP1 protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MTRDFKPGDL IFAKMKGYPH WPARVDEVPD GAVKPPTNKL PIFFFGTHET AFLGPKDIFP YSENKEKYGK PNKRKGFNEG LWEIDNNPKV KFSSQQASTK QSNASSDVEV EEKETNVSKE DTDQEEKASN EDVTKAVDIT TPKAARRGRK RKAEKQVDTE EAGMVTAATA SNVKASPKRG RPAATEVKIP KPRGRPKVVK QPCPSDGDMV IDEDKSKKKG PEEKQPKKQL KKEEEGQKEE EKPRKEPDKK EGKKEVESKR KNLAKPGVTS TSDSEDEDDQ EGEKKRKGGR NFQAAHRRNM LKGQHEKEAG DRKRKQEEQM ETEQQNKDEG KKPEVKKVEK KRETSMDSRL QRIHAEIKNS LKIDNLDVNR CIEALDELAS LQVTMQQAQK HTEMITTLKK IRRFKVSQVI MEKSTMLYNK FKNMFLVGEG DSVITQVLNK SLAEQRQHEE ANKTKDQGKK GPNKKLEKEP TGTKSLNGGS DAQESNHPQH NGDSNEDGKD SREASSKTKP PGEEREAEIS LKESTLDN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PSIP1 蛋白
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PC4 and SFRS1 Interacting Protein 1 (PSIP1) (AA 1-50) protein (GST tag)
PSIP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
PC4 and SFRS1 Interacting Protein 1 (PSIP1) (AA 1-530), (full length) protein (His-SUMO Tag)PSIP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
PC4 and SFRS1 Interacting Protein 1 (PSIP1) proteinPSIP1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
PC4 and SFRS1 Interacting Protein 1 (PSIP1) proteinPSIP1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
- 别名
- Psip1 (PSIP1 产品)
- 别名
- PSIP1 Protein, im:7149861 Protein, zgc:109898 Protein, LEDGF Protein, p75 Protein, PSIP2 Protein, DFS70 Protein, PAIP Protein, p52 Protein, AA408851 Protein, AU015605 Protein, Dfs70 Protein, Ledgfa Protein, Ledgfb Protein, Psip2 Protein, Ledgf Protein, SBP75 Protein, LEDGF/p75 Protein, PC4 and SFRS1 interacting protein 1 S homeolog Protein, PC4 and SFRS1 interacting protein 1 Protein, PC4 and SFRS1 interacting protein 1a Protein, LEDGF/p75 transcription factor-like Protein, psip1.S Protein, PSIP1 Protein, psip1a Protein, psip1 Protein, Psip1 Protein
- 背景
- PC4 and SFRS1-interacting protein (Lens epithelium-derived growth factor) (mLEDGF),FUNCTION: Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis (By similarity). {ECO:0000250|UniProtKB:O75475}.
- 分子量
- 59.7 kDa
- UniProt
- Q99JF8
- 途径
- Chromatin Binding, Ribonucleoprotein Complex Subunit Organization
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