PDPK1 Protein (AA 27-434) (His tag)
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- 抗原 See all PDPK1 蛋白
- PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 27-434
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PDPK1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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ASASASGSGP ASELGVPGQV DFYARFSPSP LSMKQFLDFG SVNACEKTSF MFLRQELPVR LANIMKEISL LPDNLLRTPS VQLVQSWYIQ SLQELLDFKD KSAEDAKTIY EFTDTVIRIR NRHNDVIPTM AQGVTEYKES FGVDPVTSQN VQYFLDRFYM SRISIRMLLN QHSLLFGGKG SPSHRKHIGS INPNCDVVEV IKDGYENARR LCDLYYVNSP ELELEELNAK SPGQTIQVVY VPSHLYHMVF ELFKNAMRAT MEHHADKGVY PPIQVHVTLG EEDLTVKMSD RGGGVPLRKI DRLFNYMYST APRPRVETSR AVPLAGFGYG LPISRLYAQY FQGDLKLYSL EGYGTDAVIY IKALSTESVE RLPVYNKAAW KHYKANHEAD DWCVPSREPK DMTTFRSS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Pdk1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PDPK1 蛋白
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3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-429) protein (GST tag)
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3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 92-151) protein (His-IF2DI Tag)PDPK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 351-556) protein (His-IF2DI Tag)PDPK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) protein (GST tag)PDPK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 29-436) protein (His tag)PDPK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 203-302) protein (GST tag)PDPK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 253-348) protein (GST tag)PDPK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-556) protein (Strep Tag)Crystallography grade PDPK1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
- 别名
- Pdk1 (PDPK1 产品)
- 别名
- pdpk1 Protein, MGC82080 Protein, PDK1 Protein, PDPK2 Protein, PRO0461 Protein, Pdk1 Protein, zgc:153787 Protein, 3'-phosphoinositide-dependent protein kinase 1 Protein, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Protein, ATPDK1 Protein, AtPDK1 Protein, T32M21.110 Protein, T32M21_110 Protein, zgc:77318 Protein, 3-phosphoinositide dependent protein kinase 1 Protein, 3-phosphoinositide dependent protein kinase 1 L homeolog Protein, 3-phosphoinositide dependent protein kinase-1 Protein, 3-phosphoinositide dependent protein kinase 1a Protein, 3-phosphoinositide-dependent protein kinase 1 Protein, 3'-phosphoinositide-dependent protein kinase 1 Protein, 3-phosphoinositide dependent protein kinase 1b Protein, PDPK1 Protein, pdpk1.L Protein, pdpk1 Protein, Pdpk1 Protein, pdpk1a Protein, LOC100380750 Protein, pdk-1 Protein, PDK1 Protein, pdpk1b Protein
- 背景
- Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress (By similarity). {ECO:0000250, ECO:0000269|PubMed:16517406}.
- 分子量
- 47.2 kDa Including tag.
- UniProt
- Q8BFP9
- 途径
- PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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