CPA1 Protein (AA 111-419) (His tag)
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- 抗原 See all CPA1 蛋白
- CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 111-419
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CPA1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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ALSTDAFNYA TYHTLDEIYE FMDLLVTEHP QLVSKIQIGS TFEGRPINVL KFSTGGTNRP AIWIDTGIHS REWVTQASGV WFAKKITKDY GQEPTLTAIL DNMDIFLEIV TNPDGFVYTH KTNRMWRKTR SHTEGSLCVG VDPNRNWDAA FGMPGASSNP CSETYRGKFP NSEVEVKSIV DFVTSHGNIK AFISIHSYSQ LLLYPYGYTS EPAPDKEELD QLAKSAVTAL TSLHGTKFKY GSIIDTIYQA SGSTIDWTYS QGIKYSFTFE LRDTGLRGFL LPASQIIPTA EETWLALLTI MDHTVKHPY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Cpa1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product CPA1 蛋白
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Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 293-369) protein (GST tag)
CPA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 1-419) protein (GST tag)CPA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carboxypeptidase A1 (Pancreatic) (CPA1) (Active) protein (His tag)CPA1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 111-419) protein (His tag)Crystallography grade CPA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Carboxypeptidase A1 (Pancreatic) (CPA1) protein (Myc-DYKDDDDK Tag)CPA1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))
- 别名
- Cpa1 (CPA1 产品)
- 别名
- cpa1 Protein, cbpa1 Protein, CPA Protein, CPA1 Protein, 0910001L12Rik Protein, Cpa Protein, carboxypeptidase A1 Protein, pancreatic carboxypeptidase A1 Protein, Carboxypeptidase A1 Protein, carboxypeptidase A1-like Protein, carboxypeptidase a1 Protein, carboxypeptidase A1, pancreatic Protein, CpipJ_CPIJ007169 Protein, CpipJ_CPIJ010802 Protein, CpipJ_CPIJ010805 Protein, MCYG_06789 Protein, MGYG_03840 Protein, cpa1 Protein, cbpa1 Protein, LOC100224866 Protein, CPA1 Protein, Cpa1 Protein
- 背景
- Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro. {ECO:0000250}.
- 分子量
- 35.6 kDa Including tag.
- UniProt
- Q7TPZ8
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