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Gasdermin A3 Protein (GSDMA3) (AA 1-464) (Strep Tag)

Crystallography grade GSDMA3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
产品编号 ABIN3122670
发货至: 中国
  • 抗原
    Gasdermin A3 (GSDMA3)
    蛋白类型
    Recombinant
    产品特性
    AA 1-464
    宿主
    小鼠
    资源
    Tobacco (Nicotiana tabacum)
    标记
    This Gasdermin A3 protein is labelled with Strep Tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    序列
    MPVFEDVTRA LVRELNPRGD LTPLDSLIDF KHFRPFCLVL RKRKSTLFWG ARYVRTDYTL LDLLEPGSSP SDLTDSGNFS FKNMLDVQVQ GLVEVPKTVK VKGTAGLSQS STLEVQTLSV APSALENLKK ERKLSADHSF LNEMRYHEKN LYVVMEAVEA KQEVTVEQTG NANAIFSLPS LALLGLQGSL NNNKAVTIPK GCVLAYRVRL LRVFLFNLWD IPYICNDSMQ TFPKIRRVPC SAFISPTQMI SEEPEEEKLI GEMHEDFKTL KEEVQRETQE VEKLSPVGRS SLLTSLSHLL GKKKELQDLE QKLEGALDKG QKVTLEALPK DVLLSKDAMD AILYFLGALT ELTEEQLKIL VKSLEKKILP VQLKLVESTL EQNFLQDKEG VFPLQPDLLS SLGEEELTLT EALVGLSGLE VQRSGPQYAW DPDTRHNLCA LYAGLSLLHL LSRKSNALTY CALS
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    Gasdermin A3 (GSDMA3)
    别名
    Gsdma3
    别名
    Bsk Protein, Dfl Protein, Fgn Protein, Gsdm1l Protein, Gsdm3 Protein, Rco2 Protein, Rim3 Protein, gasdermin A3 Protein, Gsdma3 Protein
    背景
    Gasdermin-A3 (Gasdermin-3) [Cleaved into: Gasdermin-A3, N-terminal (GSDMA3-NT), Gasdermin-A3, C-terminal (GSDMA3-CT)],FUNCTION: [Gasdermin-A3]: Precursor of a pore-forming protein involved in the transition from catagen to telogen at the end of hair follicle morphogenesis (PubMed:15475261, PubMed:26375003, PubMed:27281216). This form constitutes the precursor of the pore: upon cleavage, the released N-terminal moiety (Gasdermin-A3, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:26375003, PubMed:27281216, PubMed:35545613). This form acts as a sensor of infection: activation is triggered by cleavage by some bacterial effector protein, which releases the N-terminal moiety (Gasdermin-A3, N-terminal) (By similarity). {ECO:0000250|UniProtKB:Q9EST1, ECO:0000269|PubMed:15475261, ECO:0000269|PubMed:26375003, ECO:0000269|PubMed:27281216, ECO:0000269|PubMed:35545613}., FUNCTION: [Gasdermin-A3, N-terminal]: Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed:26375003, PubMed:27281216, PubMed:35545613). Released upon cleavage by some bacterial effector protein, and binds to membrane inner leaflet lipids (By similarity). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukin-1 (IL1B and IL18) and triggering pyroptosis (PubMed:27281216, PubMed:33883744, PubMed:35545613). Binds to membrane inner leaflet lipids, including bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to monophosphorylated phosphatidylinositols (PubMed:27281216). Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216, PubMed:29695864). Plays a role in the transition from catagen to telogen at the end of hair follicle morphogenesis, possibly by regulating hair follicle stem cell niche maintenance (PubMed:15475261, PubMed:15737203, PubMed:17572385, PubMed:22155111, PubMed:32302611). Also required for mammary gland development (PubMed:28168650). {ECO:0000250|UniProtKB:Q9EST1, ECO:0000269|PubMed:15475261, ECO:0000269|PubMed:15737203, ECO:0000269|PubMed:17572385, ECO:0000269|PubMed:22155111, ECO:0000269|PubMed:26375003, ECO:0000269|PubMed:27281216, ECO:0000269|PubMed:28168650, ECO:0000269|PubMed:29695864, ECO:0000269|PubMed:32302611, ECO:0000269|PubMed:33883744, ECO:0000269|PubMed:35545613}.
    分子量
    52.0 kDa
    UniProt
    Q5Y4Y6
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