DTYMK Protein (AA 2-212) (His tag)
-
- 抗原 See all DTYMK 蛋白
- DTYMK (Deoxythymidylate Kinase (Thymidylate Kinase) (DTYMK))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-212
-
宿主
- 小鼠
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This DTYMK protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
-
ASRRGALIVL EGVDRAGKTT QGLKLVTALC ASGHRAELLR FPERSTEIGK LLNSYLEKKT ELEDHSVHLL FSANRWEQVP LIKAKLNQGV TLVLDRYAFS GVAFTGAKEN FSLDWCKQPD VGLPKPDLIL FLQLQLLDAA ARGEFGLERY ETGTFQKQVL LCFQQLMEEK NLNWKVVDAS KSIEEVHKEI RAHSEDAIRN AAQRPLGELW K
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Dtymk Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product DTYMK 蛋白
-
Deoxythymidylate Kinase (Thymidylate Kinase) (DTYMK) (AA 1-212) protein (His tag)
DTYMK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Deoxythymidylate Kinase (Thymidylate Kinase) (DTYMK) (AA 1-212) protein (GST tag)DTYMK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Deoxythymidylate Kinase (Thymidylate Kinase) (DTYMK) (AA 103-212) protein (GST tag)DTYMK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Deoxythymidylate Kinase (Thymidylate Kinase) (DTYMK) protein (Myc-DYKDDDDK Tag)DTYMK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- DTYMK (Deoxythymidylate Kinase (Thymidylate Kinase) (DTYMK))
- 别名
- Dtymk (DTYMK 产品)
- 别名
- tymk Protein, wu:fa12c02 Protein, wu:fb14h07 Protein, wu:ft84f02 Protein, zgc:123174 Protein, DTYMK Protein, MGC81047 Protein, Tb08.28L1.680 Protein, Tb08.10J17.310 Protein, DDBDRAFT_0188698 Protein, DDBDRAFT_0230101 Protein, DDB_0188698 Protein, DDB_0230101 Protein, AU044245 Protein, CDC8 Protein, D10Ertd203e Protein, TMPK Protein, TYPK Protein, Tmk Protein, mtmk Protein, PP3731 Protein, TYMK Protein, deoxythymidylate kinase Protein, deoxythymidylate kinase (thymidylate kinase) Protein, deoxythymidylate kinase S homeolog Protein, thymidylate kinase Protein, dTMP kinase Protein, Dtymk Protein, dtymk Protein, DTYMK Protein, dtymk.S Protein, Tmk Protein, CNC04630 Protein, Tb927.8.3510 Protein, Tb927.8.3910 Protein, Ndas_4954 Protein, Mesil_2461 Protein, Slip_0046 Protein, Trad_0568 Protein, Olsu_1446 Protein, Acear_0042 Protein, Igag_1070 Protein, LOC100195817 Protein
- 背景
- Catalyzes the conversion of dTMP to dTDP.
- 分子量
- 24.7 kDa Including tag.
- UniProt
- P97930
- 途径
- Nucleotide Phosphorylation
-
