G3BP1 Protein (AA 2-465) (His tag)
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- 抗原 See all G3BP1 蛋白
- G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-465
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This G3BP1 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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VMEKPSPLLV GREFVRQYYT LLNQAPDMLH RFYGKNSSYA HGGLDSNGKP ADAVYGQKEI HRKVMSQNFT NCHTKIRHVD AHATLNDGVV VQVMGLLSNN NQALRRFMQT FVLAPEGSVA NKFYVHNDIF RYQDEVFGGF VTEPQEESEE EVEEPEERQQ TPEVVPDDSG TFYDQTVSND LEEHLEEPVV EPEPEPEPEP EPEPVSDIQE DKPEAALEEA APDDVQKSTS PAPADVAPAQ EDLRTFSWAS VTSKNLPPSG AVPVTGTPPH VVKVPASQPR PESKPDSQIP PQRPQRDQRV REQRINIPPQ RGPRPIREAG EPGDVEPRRM VRHPDSHQLF IGNLPHEVDK SELKDFFQNF GNVVELRINS GGKLPNFGFV VFDDSEPVQK VLSNRPIMFR GAVRLNVEEK KTRAAREGDR RDNRLRGPGG PRGGPSGGMR GPPRGGMVQK PGFGVGRGIT TPRQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse G3bp1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product G3BP1 蛋白
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GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) (AA 278-466) protein (His-IF2DI Tag)
G3BP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) (AA 1-466) protein (GST tag)G3BP1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) (AA 214-303) protein (GST tag)G3BP1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) (AA 1-466) protein (His tag)G3BP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) (AA 2-466) protein (His tag)Crystallography grade G3BP1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)G3BP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)G3BP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))
- 别名
- G3bp1 (G3BP1 产品)
- 别名
- G3BP Protein, HDH-VIII Protein, fj17h05 Protein, zgc:56034 Protein, wu:fj17h05 Protein, g3bp Protein, MGC53271 Protein, G3BP1 Protein, G3bp Protein, RGD1310666 Protein, AI849976 Protein, B430204O07 Protein, C87777 Protein, mKIAA4115 Protein, G3BP stress granule assembly factor 1 Protein, GTPase activating protein (SH3 domain) binding protein 1 Protein, GTPase activating protein (SH3 domain) binding protein 1 L homeolog Protein, Ras-GTPase-activating protein SH3-domain-binding protein Protein, G3BP1 Protein, g3bp1 Protein, g3bp1.L Protein, LOC100304885 Protein, G3bp1 Protein
- 背景
- May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
- 分子量
- 52.7 kDa Including tag.
- UniProt
- P97855
- 途径
- SARS-CoV-2 Protein Interactome
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