LGR4 Protein (AA 25-951) (rho-1D4 tag)
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- 抗原 See all LGR4 蛋白
- LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-951
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宿主
- 人
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资源
- Insect Cells
- 标记
- This LGR4 protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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APPLCAAPCS CDGDRRVDCS GKGLTAVPEG LSAFTQALDI SMNNITQLPE DAFKNFPFLE ELQLAGNDLS FIHPKALSGL KELKVLTLQN NQLKTVPSEA IRGLSALQSL RLDANHITSV PEDSFEGLVQ LRHLWLDDNS LTEVPVHPLS NLPTLQALTL ALNKISSIPD FAFTNLSSLV VLHLHNNKIR SLSQHCFDGL DNLETLDLNY NNLGEFPQAI KALPSLKELG FHSNSISVIP DGAFDGNPLL RTIHLYDNPL SFVGNSAFHN LSDLHSLVIR GASMVQQFPN LTGTVHLESL TLTGTKISSI PNNLCQEQKM LRTLDLSYNN IRDLPSFNGC HALEEISLQR NQIYQIKEGT FQGLISLRIL DLSRNLIHEI HSRAFATLGP ITNLDVSFNE LTSFPTEGLN GLNQLKLVGN FKLKEALAAK DFVNLRSLSV PYAYQCCAFW GCDSYANLNT EDNSLQDHSV AQEKGTADAA NVTSTLENEE HSQIIIHCTP STGAFKPCEY LLGSWMIRLT VWFIFLVALF FNLLVILTTF ASCTSLPSSK LFIGLISVSN LFMGIYTGIL TFLDAVSWGR FAEFGIWWET GSGCKVAGFL AVFSSESAIF LLMLATVERS LSAKDIMKNG KSNHLKQFRV AALLAFLGAT VAGCFPLFHR GEYSASPLCL PFPTGETPSL GFTVTLVLLN SLAFLLMAVI YTKLYCNLEK EDLSENSQSS MIKHVAWLIF TNCIFFCPVA FFSFAPLITA ISISPEIMKS VTLIFFPLPA CLNPVLYVFF NPKFKEDWKL LKRRVTKKSG SVSVSISSQG GCLEQDFYYD CGMYSHLQGN LTVCDCCESF LLTKPVSCKH LIKSHSCPAL AVASCQRPEG YWSDCGTQSA HSDYADEEDS FVSDSSDQVQ ACGRACFYQS RGFPLVRYAY NLPRVKD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human LGR4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product LGR4 蛋白
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Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 852-950) protein (GST tag)
LGR4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 805-951) protein (His tag)Crystallography grade LGR4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 805-951) protein (His tag)Crystallography grade LGR4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 25-544) protein (His tag)Crystallography grade LGR4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 25-544) protein (His tag)Crystallography grade LGR4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 25-951) protein (rho-1D4 tag)Crystallography grade LGR4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 25-544) (Active) protein (His tag)LGR4 宿主: 人 宿主: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))
- 别名
- LGR4 (LGR4 产品)
- 别名
- BNMD17 Protein, GPR48 Protein, 9130225G07 Protein, A330106J01Rik Protein, A930009A08Rik Protein, Gpr48 Protein, leucine rich repeat containing G protein-coupled receptor 4 Protein, leucine-rich repeat-containing G protein-coupled receptor 4 Protein, LGR4 Protein, Lgr4 Protein
- 背景
- Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development, required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (By similarity). {ECO:0000250|UniProtKB:A2ARI4, ECO:0000269|PubMed:21693646, ECO:0000269|PubMed:21727895, ECO:0000269|PubMed:21909076, ECO:0000269|PubMed:22815884, ECO:0000269|PubMed:23444378, ECO:0000269|PubMed:23756652}.
- 分子量
- 103.5 kDa Including tag.
- UniProt
- Q9BXB1
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