NrCAM Protein (AA 25-1304) (rho-1D4 tag)
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- 抗原 See all NrCAM (NRCAM) 蛋白
- NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-1304
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宿主
- 人
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资源
- Insect Cells
- 标记
- This NrCAM protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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QMISALEVPL DPKLLEDLVQ PPTITQQSPK DYIIDPRENI VIQCEAKGKP PPSFSWTRNG THFDIDKDPL VTMKPGTGTL IINIMSEGKA ETYEGVYQCT ARNERGAAVS NNIVVRPSRS PLWTKEKLEP ITLQSGQSLV LPCRPPIGLP PPIIFWMDNS FQRLPQSERV SQGLNGDLYF SNVLPEDTRE DYICYARFNH TQTIQQKQPI SVKVISVDEL NDTIAANLSD TEFYGAKSSR ERPPTFLTPE GNASNKEELR GNVLSLECIA EGLPTPIIYW AKEDGMLPKN RTVYKNFEKT LQIIHVSEAD SGNYQCIAKN ALGAIHHTIS VRVKAAPYWI TAPQNLVLSP GEDGTLICRA NGNPKPRISW LTNGVPIEIA PDDPSRKIDG DTIIFSNVQE RSSAVYQCNA SNEYGYLLAN AFVNVLAEPP RILTPANTLY QVIANRPALL DCAFFGSPLP TIEWFKGAKG SALHEDIYVL HENGTLEIPV AQKDSTGTYT CVARNKLGMA KNEVHLEIKD PTWIVKQPEY AVVQRGSMVS FECKVKHDHT LSLTVLWLKD NRELPSDERF TVDKDHLVVA DVSDDDSGTY TCVANTTLDS VSASAVLSVV APTPTPAPVY DVPNPPFDLE LTDQLDKSVQ LSWTPGDDNN SPITKFIIEY EDAMHKPGLW HHQTEVSGTQ TTAQLKLSPY VNYSFRVMAV NSIGKSLPSE ASEQYLTKAS EPDKNPTAVE GLGSEPDNLV ITWKPLNGFE SNGPGLQYKV SWRQKDGDDE WTSVVVANVS KYIVSGTPTF VPYLIKVQAL NDMGFAPEPA VVMGHSGEDL PMVAPGNVRV NVVNSTLAEV HWDPVPLKSI RGHLQGYRIY YWKTQSSSKR NRRHIEKKIL TFQGSKTHGM LPGLEPFSHY TLNVRVVNGK GEGPASPDRV FNTPEGVPSA PSSLKIVNPT LDSLTLEWDP PSHPNGILTE YTLKYQPINS THELGPLVDL KIPANKTRWT LKNLNFSTRY KFYFYAQTSA GSGSQITEEA VTTVDEAGIL PPDVGAGKVQ AVNPRISNLT AAAAETYANI SWEYEGPEHV NFYVEYGVAG SKEEWRKEIV NGSRSFFGLK GLMPGTAYKV RVGAVGDSGF VSSEDVFETG PAMASRQVDI ATQGWFIGLM CAVALLILIL LIVCFIRRNK GGKYPVKEKE DAHADPEIQP MKEDDGTFGE YSDAEDHKPL KKGSRTPSDR TVKKEDSDDS LVDYGEGVNG QFNEDGSFIG QYSGKKEKEP AEGNESSEAP SPVNAMNSFV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human NRCAM Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product NRCAM 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))
- 别名
- NRCAM (NRCAM 产品)
- 别名
- CD56 Protein, MSK39 Protein, NCAM Protein, Bravo Protein, C030017F07Rik Protein, C130076O07Rik Protein, mKIAA0343 Protein, NRCAM Protein, si:dkey-240a12.1 Protein, Nr-CAM Protein, neural cell adhesion molecule 1 Protein, neuronal cell adhesion molecule Protein, neuronal cell adhesion molecule L homeolog Protein, neuronal cell adhesion molecule a Protein, NCAM1 Protein, NRCAM Protein, Nrcam Protein, nrcam.L Protein, nrcama Protein, nrcam Protein
- 背景
- Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes, not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier. {ECO:0000250|UniProtKB:Q810U4}.
- 分子量
- 142.3 kDa Including tag.
- UniProt
- Q92823
- 途径
- Regulation of Cell Size
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