NMDAR2A Protein (AA 23-1464) (rho-1D4 tag)
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- 抗原 See all NMDAR2A (GRIN2A) 蛋白
- NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-1464
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宿主
- 人
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资源
- Insect Cells
- 标记
- This NMDAR2A protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
- 序列
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PSAAAEKGPP ALNIAVMLGH SHDVTERELR TLWGPEQAAG LPLDVNVVAL LMNRTDPKSL ITHVCDLMSG ARIHGLVFGD DTDQEAVAQM LDFISSHTFV PILGIHGGAS MIMADKDPTS TFFQFGASIQ QQATVMLKIM QDYDWHVFSL VTTIFPGYRE FISFVKTTVD NSFVGWDMQN VITLDTSFED AKTQVQLKKI HSSVILLYCS KDEAVLILSE ARSLGLTGYD FFWIVPSLVS GNTELIPKEF PSGLISVSYD DWDYSLEARV RDGIGILTTA ASSMLEKFSY IPEAKASCYG QMERPEVPMH TLHPFMVNVT WDGKDLSFTE EGYQVHPRLV VIVLNKDREW EKVGKWENHT LSLRHAVWPR YKSFSDCEPD DNHLSIVTLE EAPFVIVEDI DPLTETCVRN TVPCRKFVKI NNSTNEGMNV KKCCKGFCID ILKKLSRTVK FTYDLYLVTN GKHGKKVNNV WNGMIGEVVY QRAVMAVGSL TINEERSEVV DFSVPFVETG ISVMVSRSNG TVSPSAFLEP FSASVWVMMF VMLLIVSAIA VFVFEYFSPV GYNRNLAKGK APHGPSFTIG KAIWLLWGLV FNNSVPVQNP KGTTSKIMVS VWAFFAVIFL ASYTANLAAF MIQEEFVDQV TGLSDKKFQR PHDYSPPFRF GTVPNGSTER NIRNNYPYMH QYMTKFNQKG VEDALVSLKT GKLDAFIYDA AVLNYKAGRD EGCKLVTIGS GYIFATTGYG IALQKGSPWK RQIDLALLQF VGDGEMEELE TLWLTGICHN EKNEVMSSQL DIDNMAGVFY MLAAAMALSL ITFIWEHLFY WKLRFCFTGV CSDRPGLLFS ISRGIYSCIH GVHIEEKKKS PDFNLTGSQS NMLKLLRSAK NISSMSNMNS SRMDSPKRAA DFIQRGSLIM DMVSDKGNLM YSDNRSFQGK ESIFGDNMNE LQTFVANRQK DNLNNYVFQG QHPLTLNESN PNTVEVAVST ESKANSRPRQ LWKKSVDSIR QDSLSQNPVS QRDEATAENR THSLKSPRYL PEEMAHSDIS ETSNRATCHR EPDNSKNHKT KDNFKRSVAS KYPKDCSEVE RTYLKTKSSS PRDKIYTIDG EKEPGFHLDP PQFVENVTLP ENVDFPDPYQ DPSENFRKGD STLPMNRNPL HNEEGLSNND QYKLYSKHFT LKDKGSPHSE TSERYRQNST HCRSCLSNMP TYSGHFTMRS PFKCDACLRM GNLYDIDEDQ MLQETGNPAT GEQVYQQDWA QNNALQLQKN KLRISRQHSY DNIVDKPREL DLSRPSRSIS LKDRERLLEG NFYGSLFSVP SSKLSGKKSS LFPQGLEDSK RSKSLLPDHT SDNPFLHSHR DDQRLVIGRC PSDPYKHSLP SQAVNDSYLR SSLRSTASYC SRDSRGHNDV YISEHVMPYA ANKNNMYSTP RVLNSCSNRR VYKKMPSIES DV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human GRIN2A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product GRIN2A 蛋白
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Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 655-816) protein (His tag)
Crystallography grade GRIN2A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 655-816) protein (His tag)Crystallography grade GRIN2A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 23-555) protein (His tag)Crystallography grade GRIN2A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 838-1464) protein (His tag)Crystallography grade GRIN2A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 23-555) protein (His tag)Crystallography grade GRIN2A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 838-1464) protein (His tag)Crystallography grade GRIN2A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 23-1464) protein (rho-1D4 tag)Crystallography grade GRIN2A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))
- 别名
- GRIN2A (GRIN2A 产品)
- 别名
- NMDAR2A Protein, nr2a Protein, GRIN2A Protein, EPND Protein, GluN2A Protein, NR2A Protein, glutamate ionotropic receptor NMDA type subunit 2A Protein, glutamate receptor, ionotropic, N-methyl D-aspartate 2A L homeolog Protein, glutamate receptor ionotropic, NMDA 2A Protein, glutamate receptor, ionotropic, NMDA2A (epsilon 1) Protein, GRIN2A Protein, grin2a.L Protein, LOC100438553 Protein, Grin2a Protein
- 背景
- NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
- 分子量
- 164.0 kDa Including tag.
- UniProt
- Q12879
- 途径
- Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity
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