KLRC1 Protein (AA 1-233) (Strep Tag)
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北京 101111
Quick Overview for KLRC1 Protein (AA 1-233) (Strep Tag) (ABIN3097102)
抗原
See all KLRC1 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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产品特性
- AA 1-233
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标记
- This KLRC1 protein is labelled with Strep Tag.
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序列
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MDNQGVIYSD LNLPPNPKRQ QRKPKGNKNS ILATEQEITY AELNLQKASQ DFQGNDKTYH CKDLPSAPEK LIVGILGIIC LILMASVVTI VVIPSTLIQR HNNSSLNTRT QKARHCGHCP EEWITYSNSC YYIGKERRTW EESLLACTSK NSSLLSIDNE EEMKFLSIIS PSSWIGVFRN SSHHPWVTMN GLAFKHEIKD SDNAELNCAV LQVNRLKSAQ CGSSIIYHCK HKL
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.. -
产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
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纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 HEK-293 CellsConjugate Biotin,His-Avi TagOrigin HumanPrice 6,261.95 ¥表达系统 HEK-293 CellsConjugate His-Avi TagOrigin HumanPrice 3,956.76 ¥表达系统 HEK-293 CellsConjugate hFc TagOrigin HumanPrice 3,956.76 ¥表达系统 HEK-293 CellsConjugate Fc TagOrigin HumanPrice 7,574.17 ¥表达系统 Wheat germConjugate GST tagOrigin HumanPrice 5,782.24 ¥表达系统 Baculovirus infected Insect CellsConjugate His tagOrigin HumanPrice 15,253.89 ¥表达系统 Human CellsConjugate His tagOrigin HumanPrice 1,886.64 ¥表达系统 Mammalian CellsConjugate His tagOrigin HumanPrice 2,380.07 ¥表达系统 Wheat germConjugateOrigin HumanPrice 8,510.26 ¥表达系统 HEK-293 CellsConjugate His tagOrigin HumanPrice 4,475.67 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1) (AA 100-233) (Active) protein (Biotin,His-Avi Tag)
KLRC1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1) (AA 100-233) (Active) protein (His-Avi Tag)KLRC1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1) (AA 100-219) (Active) protein (hFc Tag)KLRC1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1) (AA 1-233) protein (His tag)custom-made KLRC1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1) (AA 99-330) protein (Fc Tag)KLRC1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1) (AA 1-233) protein (GST tag)KLRC1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1) protein (His tag)KLRC1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1) (AA 94-233) protein (His tag)KLRC1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. -
注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- KLRC1 (Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))
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别名
- KLRC1
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背景
- NKG2-A/NKG2-B type II integral membrane protein (CD159 antigen-like family member A) (NK cell receptor A) (NKG2-A/B-activating NK receptor) (CD antigen CD159a),FUNCTION: Immune inhibitory receptor involved in self-nonself discrimination. In complex with KLRD1 on cytotoxic and regulatory lymphocyte subsets, recognizes non-classical major histocompatibility (MHC) class Ib molecule HLA-E loaded with self-peptides derived from the signal sequence of classical MHC class Ia molecules. Enables cytotoxic cells to monitor the expression of MHC class I molecules in healthy cells and to tolerate self (PubMed:9486650, PubMed:18083576, PubMed:9430220, PubMed:37264229). Upon HLA-E-peptide binding, transmits intracellular signals through two immunoreceptor tyrosine-based inhibition motifs (ITIMs) by recruiting INPP5D/SHP-1 and INPPL1/SHP-2 tyrosine phosphatases to ITIMs, and ultimately opposing signals transmitted by activating receptors through dephosphorylation of proximal signaling molecules (PubMed:9485206, PubMed:12165520). Key inhibitory receptor on natural killer (NK) cells that regulates their activation and effector functions (PubMed:9486650, PubMed:9430220, PubMed:9485206, PubMed:30860984). Dominantly counteracts T cell receptor signaling on a subset of memory/effector CD8-positive T cells as part of an antigen-driven response to avoid autoimmunity (PubMed:12387742). On intraepithelial CD8-positive gamma-delta regulatory T cells triggers TGFB1 secretion, which in turn limits the cytotoxic programming of intraepithelial CD8-positive alpha-beta T cells, distinguishing harmless from pathogenic antigens (PubMed:18064301). In HLA-E-rich tumor microenvironment, acts as an immune inhibitory checkpoint and may contribute to progressive loss of effector functions of NK cells and tumor-specific T cells, a state known as cell exhaustion (PubMed:30503213, PubMed:30860984). {ECO:0000269|PubMed:12165520, ECO:0000269|PubMed:12387742, ECO:0000269|PubMed:18064301, ECO:0000269|PubMed:18083576, ECO:0000269|PubMed:30503213, ECO:0000269|PubMed:30860984, ECO:0000269|PubMed:37264229, ECO:0000269|PubMed:9430220, ECO:0000269|PubMed:9485206, ECO:0000269|PubMed:9486650}., FUNCTION: (Microbial infection) Viruses like human cytomegalovirus have evolved an escape mechanism whereby virus-induced down-regulation of host MHC class I molecules is coupled to the binding of viral peptides to HLA-E, restoring HLA-E expression and inducing HLA-E-dependent NK cell immune tolerance to infected cells. Recognizes HLA-E in complex with human cytomegalovirus UL40-derived peptide (VMAPRTLIL) and inhibits NK cell cytotoxicity. {ECO:0000269|PubMed:10669413, ECO:0000269|PubMed:23335510}., FUNCTION: (Microbial infection) May recognize HLA-E in complex with HIV-1 gag/Capsid protein p24-derived peptide (AISPRTLNA) on infected cells and may inhibit NK cell cytotoxicity, a mechanism that allows HIV-1 to escape immune recognition. {ECO:0000269|PubMed:15751767}., FUNCTION: (Microbial infection) Upon SARS-CoV-2 infection, may contribute to functional exhaustion of cytotoxic NK cells and CD8-positive T cells (PubMed:32203188, PubMed:32859121). On NK cells, may recognize HLA-E in complex with SARS-CoV-2 S/Spike protein S1-derived peptide (LQPRTFLL) expressed on the surface of lung epithelial cells, inducing NK cell exhaustion and dampening antiviral immune surveillance (PubMed:32859121). {ECO:0000269|PubMed:32203188, ECO:0000269|PubMed:32859121}.
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分子量
- 26.3 kDa
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UniProt
- P26715
抗原
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