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SYNPO Protein (AA 1-929) (His tag)

Crystallography grade SYNPO 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
产品编号 ABIN3095768
发货至: 中国
  • 抗原 See all SYNPO 蛋白
    SYNPO (Synaptopodin (SYNPO))
    蛋白类型
    Recombinant
    产品特性
    AA 1-929
    宿主
    • 4
    • 1
    资源
    • 3
    • 1
    • 1
    Insect Cells
    标记
    This SYNPO protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    序列
    MLGPHLPPPP LAPSEGRPTP CAFQIPDGSY RCLALEAEES SGEEGLQGEV GPTDLEEDEG VSRSGDDSAC RVTQGTPQLP KALGIQPPSC SREEQGASQH DDRASQDWDV VKAGQMMTAS PSPGPGPRVA QKPALGRSTS LTEKDLKEAK ARSQQIAAQL TTPPSSNSRG VQLFNRRRQR VNEFTLESHG QRGQKPSQES LRVLPSSLPG HAPGLSLSST SLPEPGPPRH PSPQSPDRGV PGHSMEGYSE EASLLRHLEK VASEEEEVPL VVYLKENAAL LTANGLHLSQ NREAQQSSPA PPPAEVHSPA ADVNQNLASP SATLTTPTSN SSHNPPATDV NQNPPATVVP QSLPLSSIQQ NSSEAQLPSN GTGPASKPST LCADGQPQAP AEEVRCSTLL IDKVSTPATT TSTFSREATL IPSSRPPASD FMSSSLLIDI QPNTLVVSAD QEMSGRAAAT TPTKVYSEVH FTLAKPPSVV NRTARPFGIQ APGGTSQMER SPMLERRHFG EKAPAPQPPS LPDRSPRPQR HIMSRSPMVE RRMMGQRSPA SERRPLGNFT APPTYTETLS TAPLASWVRS PPSYSVLYPS SDPKSSHLKG QAVPASKTGI LEESMARRGS RKSMFTFVEK PKVTPNPDLL DLVQTADEKR RQRDQGEVGV EEEPFALGAE ASNFQQEPAP RDRASPAAAE EVVPEWASCL KSPRIQAKPK PKPNQNLSEA SGKGAELYAR RQSRMEKYVI ESSSHTPELA RCPSPTMSLP SSWKYPTNAP GAFRVASRSP ARTPPASLYH GYLPENGVLR PEPTKQPPYQ LRPSLFVLSP IKEPAKVSPR AASPAKPSSL DLVPNLPKGA LPPSPALPRP SRSSPGLYTS PGQDSLQPTA VSPPYGGDIS PVSPSRAWSP RAKQAPRPSF STRNAGIEAQ VWKPSFCFK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SYNPO Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    SYNPO (Synaptopodin (SYNPO))
    别名
    SYNPO (SYNPO 产品)
    别名
    SYNPO Protein, DKFZp459N012 Protein, 9030217H17Rik Protein, 9130229N11 Protein, 9330140I15Rik Protein, AW046661 Protein, synaptopodin Protein, formin-like protein 5 Protein, SYNPO Protein, LOC100147046 Protein, Synpo Protein
    背景
    Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity (By similarity). {ECO:0000250}.
    分子量
    100.4 kDa Including tag.
    UniProt
    Q8N3V7
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