SSC5D Protein (AA 17-1573) (His tag)
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- 抗原 See all SSC5D products
- SSC5D (Scavenger Receptor Cysteine Rich Domain Containing (5 Domains) (SSC5D))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-1573
- 宿主
- 人
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资源
- Insect Cells
- 标记
- This SSC5D protein is labelled with His tag.
- 应用范围
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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VERLRLADGP HGCAGRLEVW HGGRWGTVCD DGWDLRDAAV ACRQLGCGGA LAAPGGAFFG EGAGPVWLSE LACRGNEGQL GLCHHRGWKA HICSHEEDAG VVCAGQRVAN SRDDSTSPLD GAPWPGLLLE LSPSTEEPLV THAPRPAGNP QNASRKKSPR PKQAKSTRAP LLTTGAPRQE RLRLVSGPHR CAGRLEVWHG GRWGTVCDDG WDLRDAAVAC RELGCGGALA APGGARFGPG AGPVWMDDVG CGGGEQALRD CPRSPWGRSN CDHSEDAGLV CTGPAPRLRL ADGPHGCAGR LEVWHGGRWG SVCDDAWDLR DAAVACRELG CGGALAAPGG AFFGEGSGPI ILDDLRCRGN ETALRFCPAR PWGQHDCHHR EDAGAVCDGM PLGYVPPTAP TDSNNSTPRE AASRPPSTMT SQAPGTAGVS PPPASPTVLW EPGPEAGSPQ LRLVAGPSKC SGRLEVWHDQ RWGTVCDDSW DMRDSAVVCR ELGCGGPQQP DPAAGRFGWG AGPIWLDDVG CVGTEASLSD CPAAPWGKHN CAHNEDVGVT CTGPPGLDSI SDPFSWSWIP GLGRDRDAWL PGELATKPSA SVTASVLEKT TTKAPGKMPK STKKWVTKNA KRPTTQPPVM PTTKHSRAQS PPDLTSQTTA ALTTEASRRP TSEFTRRPTT EAPQRWTSHT TATLTPQAPR ERTTKTMAML TTQGPQEMTS ESTIKSIPQA SLEPSAEIPE GSPESPKDPA PSPSVSTTGE SGLFRVRLAD GPNRCAGRLE VWHAGRWGTV CDDNWDLRDA TVACWELGCG KVRPRVGKTH YGPGTGPIWL DDMGCKGSEA SLSDCPSGAW GKHNCDHEED VGLTCTGYTD YDDYPPWTWD PTSREDLAKG TTTAGVPGHT LPWRTTRRPG SSSPAIRRLP DTGSKDGYKL PWTWDTPSGR GLAEGTPTAG KLGPTLGAGT TRSPGSPPTL RVHGDTGSPR KPWPERRPPR PAATRTAPPT PSPGPSASPG PPGPALTSDS SRELTPHSAL TSEATSDAPD TSPPTPDPAS RTNPDLILTS PDFALSTPDS SVVPALTPEP SPTPLPTLPK ELTSDPSTPS EVTSLSPTSE QVPESDTTPD LDTTPYSSTV SEYSRSPDPS PSPHPTTTPD PTMAPDPITT LNPTVTPHFP TTPHPTTTPH PTTITHSTMI PDPTTTPQPF TTITHSTMIP DPTTTPQPFT TMQPTTTPHS TTPHPTTTPH PTTITHSTMI PDPTTTPQPF TTMQPTTMPH PTTTPHPTTT PHPTTTPHPT TTPHPTMTPD PTTTPYPTTT PDPTTTPHPT TPDPSSTPVI TTVSLPTSLG TELSSPTLAP TVKPSLHPQL TFTAPAPHTS TSQIPTLEPS PALESSPSRS STATSMDPLS TEDFKPPRSQ SPNLTPPPTH TPHSASDLTV SPDPLLSPTA HPLDHPPLDP LTLGPTPGQS PGPHGPCVAP TPPVRVMACE PPALVELVAA VRDVGGQLQR LTQVVEQERQ ERQALLLGLT QLVEAARGLG QLGEAVKRLA EMAWTTSMPA PTTTTPEEEE RPLRGDV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SSC5D Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SSC5D (Scavenger Receptor Cysteine Rich Domain Containing (5 Domains) (SSC5D))
- 别名
- SSC5D (SSC5D 产品)
- 别名
- A430110N23Rik Protein, S5D-SRCRB Protein, scavenger receptor cysteine rich family member with 5 domains Protein, scavenger receptor cysteine rich family, 5 domains Protein, SSC5D Protein, Ssc5d Protein
- 背景
- Binds to extracellular matrix proteins. Binds to pathogen-associated molecular patterns (PAMPs) present on the cell walls of Gram-positive and Gram-negative bacteria and fungi, behaving as a pattern recognition receptor (PRR). Induces bacterial and fungal aggregation and subsequent inhibition of PAMP-induced cytokine release. Does not possess intrinsic bactericidal activity. May play a role in the innate defense and homeostasis of certain epithelial surfaces (By similarity). {ECO:0000250}.
- 分子量
- 165.1 kDa Including tag.
- UniProt
- A1L4H1
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