SCN9A Protein (AA 1761-1988) (His tag)
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- 抗原 See all SCN9A 蛋白
- SCN9A (Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1761-1988
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SCN9A protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS), Functional Studies (Func)
- 序列
- HHHHHHLENF SVATEESTEPL SEDDFEMFYE VWEKFDPDAT QFIEFSKLSD FAAALDPPLL IAKPNKVQLI AMDLPMVSGD RIHCLDILFA FTKRVLGESG EMDSLRSQME ERFMSANPS KVSYEPITTT LKRKQEDVSA TVIQRAYRRY RLRQNVKNIS SIYIKDGDRD DDLLNKKDMA FDNVNENSSP EKTDATSSTT SPPSYDSVTK PDKEKYEQDR TEKEDKGKDS KESKK
- 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SCN9A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount.
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
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Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 269-339) protein (GST tag)
SCN9A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 272-378) protein (His tag)Crystallography grade SCN9A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 405-737) protein (His tag)Crystallography grade SCN9A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 272-378) protein (His tag)Crystallography grade SCN9A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1759-1984) protein (His tag)Crystallography grade SCN9A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1-121) protein (His tag)Crystallography grade SCN9A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 405-738) protein (His tag)Crystallography grade SCN9A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 969-1187) protein (His tag)Crystallography grade SCN9A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1-121) protein (His tag)Crystallography grade SCN9A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 968-1185) protein (His tag)Crystallography grade SCN9A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.76 mg/mL
- 缓冲液
- 20 mM Hepes, pH 7.4; 150 mM NaCl
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SCN9A (Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A))
- 别名
- SCN9A (SCN9A 产品)
- 别名
- SCN9A Protein, DKFZp459G084 Protein, Nav1.7 Protein, PN1 Protein, mKIAA4197 Protein, Scn2a Protein, ETHA Protein, FEB3B Protein, GEFSP7 Protein, NE-NA Protein, NENA Protein, SFNP Protein, sodium channel, voltage-gated, type IX, alpha subunit Protein, sodium voltage-gated channel alpha subunit 9 Protein, sodium channel protein type 9 subunit alpha Protein, sodium channel, voltage-gated, type IX, alpha Protein, SCN9A Protein, LOC100016648 Protein, Scn9a Protein
- 背景
- Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784). {ECO:0000269|PubMed:15178348, ECO:0000269|PubMed:15385606, ECO:0000269|PubMed:16988069, ECO:0000269|PubMed:17145499, ECO:0000269|PubMed:17167479, ECO:0000269|PubMed:19369487, ECO:0000269|PubMed:24311784, ECO:0000269|PubMed:25240195, ECO:0000269|PubMed:26680203, ECO:0000269|PubMed:7720699}.
- 分子量
- 27.1 kDa Including tag.
- UniProt
- Q15858
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