Nucleolin Protein (NCL) (AA 2-710) (His tag)
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- 抗原 See all Nucleolin (NCL) 蛋白
- Nucleolin (NCL)
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-710
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宿主
- 人
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资源
- Insect Cells
- 标记
- This Nucleolin protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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VKLAKAGKNQ GDPKKMAPPP KEVEEDSEDE EMSEDEEDDS SGEEVVIPQK KGKKAAATSA KKVVVSPTKK VAVATPAKKA AVTPGKKAAA TPAKKTVTPA KAVTTPGKKG ATPGKALVAT PGKKGAAIPA KGAKNGKNAK KEDSDEEEDD DSEEDEEDDE DEDEDEDEIE PAAMKAAAAA PASEDEDDED DEDDEDDDDD EEDDSEEEAM ETTPAKGKKA AKVVPVKAKN VAEDEDEEED DEDEDDDDDE DDEDDDDEDD EEEEEEEEEE PVKEAPGKRK KEMAKQKAAP EAKKQKVEGT EPTTAFNLFV GNLNFNKSAP ELKTGISDVF AKNDLAVVDV RIGMTRKFGY VDFESAEDLE KALELTGLKV FGNEIKLEKP KGKDSKKERD ARTLLAKNLP YKVTQDELKE VFEDAAEIRL VSKDGKSKGI AYIEFKTEAD AEKTFEEKQG TEIDGRSISL YYTGEKGQNQ DYRGGKNSTW SGESKTLVLS NLSYSATEET LQEVFEKATF IKVPQNQNGK SKGYAFIEFA SFEDAKEALN SCNKREIEGR AIRLELQGPR GSPNARSQPS KTLFVKGLSE DTTEETLKES FDGSVRARIV TDRETGSSKG FGFVDFNSEE DAKAAKEAME DGEIDGNKVT LDWAKPKGEG GFGGRGGGRG GFGGRGGGRG GRGGFGGRGR GGFGGRGGFR GGRGGGGDHK PQGKKTKFE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human NCL Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
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Nucleolin (NCL) (AA 2-707) protein (His tag)
Crystallography grade NCL 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Nucleolin (NCL) protein (Myc-DYKDDDDK Tag)NCL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Nucleolin (NCL) (AA 2-482), (partial) protein (His tag)NCL 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Nucleolin (NCL)
- 别名
- NCL (NCL 产品)
- 别名
- C23 Protein, B530004O11Rik Protein, D0Nds28 Protein, D1Nds28 Protein, Nucl Protein, wu:fa12d03 Protein, zgc:152810 Protein, nucl Protein, LOC100282923 Protein, LOC100283058 Protein, nucleolin Protein, nucleolin S homeolog Protein, NCL Protein, Ncl Protein, ncl.S Protein, ncl Protein, LOC100195537 Protein, LOC100282923 Protein, LOC100283058 Protein
- 背景
- Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. {ECO:0000269|PubMed:10393184}.
- 分子量
- 77.4 kDa Including tag.
- UniProt
- P19338
- 途径
- Ribonucleoprotein Complex Subunit Organization
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