NEFM Protein (AA 2-916) (His tag)
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- 抗原 See all NEFM 蛋白
- NEFM (Neurofilament Triplet M Protein (NEFM))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-916
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宿主
- 人
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资源
- Insect Cells
- 标记
- This NEFM protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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SYTLDSLGNP SAYRRVTETR SSFSRVSGSP SSGFRSQSWS RGSPSTVSSS YKRSMLAPRL AYSSAMLSSA ESSLDFSQSS SLLNGGSGPG GDYKLSRSNE KEQLQGLNDR FAGYIEKVHY LEQQNKEIEA EIQALRQKQA SHAQLGDAYD QEIRELRATL EMVNHEKAQV QLDSDHLEED IHRLKERFEE EARLRDDTEA AIRALRKDIE EASLVKVELD KKVQSLQDEV AFLRSNHEEE VADLLAQIQA SHITVERKDY LKTDISTALK EIRSQLESHS DQNMHQAEEW FKCRYAKLTE AAEQNKEAIR SAKEEIAEYR RQLQSKSIEL ESVRGTKESL ERQLSDIEER HNHDLSSYQD TIQQLENELR GTKWEMARHL REYQDLLNVK MALDIEIAAY RKLLEGEETR FSTFAGSITG PLYTHRPPIT ISSKIQKPKV EAPKLKVQHK FVEEIIEETK VEDEKSEMEE ALTAITEELA VSMKEEKKEA AEEKEEEPEA EEEEVAAKKS PVKATAPEVK EEEGEKEEEE GQEEEEEEDE GAKSDQAEEG GSEKEGSSEK EEGEQEEGET EAEAEGEEAE AKEEKKVEEK SEEVATKEEL VADAKVEKPE KAKSPVPKSP VEEKGKSPVP KSPVEEKGKS PVPKSPVEEK GKSPVPKSPV EEKGKSPVSK SPVEEKAKSP VPKSPVEEAK SKAEVGKGEQ KEEEEKEVKE APKEEKVEKK EEKPKDVPEK KKAESPVKEE AVAEVVTITK SVKVHLEKET KEEGKPLQQE KEKEKAGGEG GSEEEGSDKG AKGSRKEDIA VNGEVEGKEE VEQETKEKGS GREEEKGVVT NGLDLSPADE KKGGDKSEEK VVVTKTVEKI TSEGGDGATK YITKSVTVTQ KVEEHEETFE EKLVSTKKVE KVTSHAIVKE VTQSD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human NEFM Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
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Neurofilament Triplet M Protein (NEFM) (AA 2-848) protein (His tag)
Crystallography grade NEFM 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Neurofilament Triplet M Protein (NEFM) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NEFM 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- NEFM (Neurofilament Triplet M Protein (NEFM))
- 别名
- NEFM (NEFM 产品)
- 别名
- NEF3 Protein, NF-M Protein, NFM Protein, Nef3 Protein, Nfm Protein, NF160 Protein, NF165 Protein, nef-m Protein, nef3 Protein, nefm Protein, nf-m Protein, zgc:112359 Protein, nefm-a Protein, nefm-b Protein, neurofilament medium Protein, neurofilament triplet M protein Protein, neurofilament, medium polypeptide Protein, neurofilament medium polypeptide Protein, neurofilament medium L homeolog Protein, neurofilament, medium polypeptide a Protein, neurofilament medium S homeolog Protein, NEFM Protein, Nefm Protein, TVAG_582010 Protein, TVAG_379630 Protein, nefm.L Protein, nefma Protein, nefm.S Protein
- 背景
- Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
- 分子量
- 103.3 kDa Including tag.
- UniProt
- P07197
- 途径
- Brown Fat Cell Differentiation
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