NDST1 Protein (AA 40-882) (His tag)
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- 抗原 See all NDST1 蛋白
- NDST1 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 40-882
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宿主
- 人
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资源
- Insect Cells
- 标记
- This NDST1 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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GWKRGLEPSA DAPEPDCGDP PPVAPSRLLP LKPVQAATPS RTDPLVLVFV ESLYSQLGQE VVAILESSRF KYRTEIAPGK GDMPTLTDKG RGRFALIIYE NILKYVNLDA WNRELLDKYC VAYGVGIIGF FKANENSLLS AQLKGFPLFL HSNLGLKDCS INPKSPLLYV TRPSEVEKGV LPGEDWTVFQ SNHSTYEPVL LAKTRSSESI PHLGADAGLH AALHATVVQD LGLHDGIQRV LFGNNLNFWL HKLVFVDAVA FLTGKRLSLP LDRYILVDID DIFVGKEGTR MKVEDVKALF DTQNELRAHI PNFTFNLGYS GKFFHTGTNA EDAGDDLLLS YVKEFWWFPH MWSHMQPHLF HNQSVLAEQM ALNKKFAVEH GIPTDMGYAV APHHSGVYPV HVQLYEAWKQ VWSIRVTSTE EYPHLKPARY RRGFIHNGIM VLPRQTCGLF THTIFYNEYP GGSSELDKII NGGELFLTVL LNPISIFMTH LSNYGNDRLG LYTFKHLVRF LHSWTNLRLQ TLPPVQLAQK YFQIFSEEKD PLWQDPCEDK RHKDIWSKEK TCDRFPKLLI IGPQKTGTTA LYLFLGMHPD LSSNYPSSET FEEIQFFNGH NYHKGIDWYM EFFPIPSNTT SDFYFEKSAN YFDSEVAPRR AAALLPKAKV LTILINPADR AYSWYQHQRA HDDPVALKYT FHEVITAGSD ASSKLRALQN RCLVPGWYAT HIERWLSAYH ANQILVLDGK LLRTEPAKVM DMVQKFLGVT NTIDYHKTLA FDPKKGFWCQ LLEGGKTKCL GKSKGRKYPE MDLDSRAFLK DYYRDHNIEL SKLLYKMGQT LPTWLREDLQ NTR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human NDST1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product NDST1 蛋白
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N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 1-882) protein (GST tag)
NDST1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 38-136) protein (GST tag)NDST1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 1-882) protein (Strep Tag)Crystallography grade NDST1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 1-882) protein (Strep Tag)Crystallography grade NDST1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 39-882) protein (His tag)Crystallography grade NDST1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 40-556), (Isoform 2) protein (His-SUMO Tag)NDST1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) protein (Myc-DYKDDDDK Tag)NDST1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- NDST1 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1))
- 别名
- NDST1 (NDST1 产品)
- 别名
- HSST Protein, NST1 Protein, 1200015G06Rik Protein, Hsst Protein, NDST-1 Protein, fj47c03 Protein, ndst1 Protein, wu:fj47c03 Protein, NDST1 Protein, N-deacetylase and N-sulfotransferase 1 Protein, N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 Protein, N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b Protein, N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 S homeolog Protein, NDST1 Protein, Ndst1 Protein, ndst1b Protein, ndst1 Protein, ndst1.S Protein
- 背景
- Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:10758005, PubMed:12634318). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (PubMed:10758005, PubMed:12634318). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000269|PubMed:10758005, ECO:0000269|PubMed:12634318, ECO:0000269|PubMed:22660413}.
- 分子量
- 97.3 kDa Including tag.
- UniProt
- P52848
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Glycosaminoglycan Metabolic Process
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