MVP Protein (AA 2-893) (His tag)
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- 抗原 See all MVP 蛋白
- MVP (Major Vault Protein (MVP))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-893
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宿主
- 人
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资源
- Insect Cells
- 标记
- This MVP protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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ATEEFIIRIP PYHYIHVLDQ NSNVSRVEVG PKTYIRQDNE RVLFAPMRMV TVPPRHYCTV ANPVSRDAQG LVLFDVTGQV RLRHADLEIR LAQDPFPLYP GEVLEKDITP LQVVLPNTAL HLKALLDFED KDGDKVVAGD EWLFEGPGTY IPRKEVEVVE IIQATIIRQN QALRLRARKE CWDRDGKERV TGEEWLVTTV GAYLPAVFEE VLDLVDAVIL TEKTALHLRA RRNFRDFRGV SRRTGEEWLV TVQDTEAHVP DVHEEVLGVV PITTLGPHNY CVILDPVGPD GKNQLGQKRV VKGEKSFFLQ PGEQLEQGIQ DVYVLSEQQG LLLRALQPLE EGEDEEKVSH QAGDHWLIRG PLEYVPSAKV EVVEERQAIP LDENEGIYVQ DVKTGKVRAV IGSTYMLTQD EVLWEKELPP GVEELLNKGQ DPLADRGEKD TAKSLQPLAP RNKTRVVSYR VPHNAAVQVY DYREKRARVV FGPELVSLGP EEQFTVLSLS AGRPKRPHAR RALCLLLGPD FFTDVITIET ADHARLQLQL AYNWHFEVND RKDPQETAKL FSVPDFVGDA CKAIASRVRG AVASVTFDDF HKNSARIIRT AVFGFETSEA KGPDGMALPR PRDQAVFPQN GLVVSSVDVQ SVEPVDQRTR DALQRSVQLA IEITTNSQEA AAKHEAQRLE QEARGRLERQ KILDQSEAEK ARKELLELEA LSMAVESTGT AKAEAESRAE AARIEGEGSV LQAKLKAQAL AIETEAELQR VQKVRELELV YARAQLELEV SKAQQLAEVE VKKFKQMTEA IGPSTIRDLA VAGPEMQVKL LQSLGLKSTL ITDGSTPINL FNTAFGLLGM GPEGQPLGRR VASGPSPGEG ISPQSAQAPQ APGDNHVVPV LR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MVP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MVP 蛋白
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Major Vault Protein (MVP) (AA 1-893) protein (GST tag)
MVP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Major Vault Protein (MVP) (AA 130-443) protein (His tag)MVP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Major Vault Protein (MVP) (AA 2-893) protein (His-SUMO Tag)MVP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Major Vault Protein (MVP) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)MVP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Major Vault Protein (MVP) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)MVP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MVP (Major Vault Protein (MVP))
- 别名
- MVP (MVP 产品)
- 别名
- LRP Protein, VAULT1 Protein, 2310009M24Rik Protein, cb771 Protein, wu:fb52b05 Protein, wu:fc02g01 Protein, mvp Protein, MGC145641 Protein, Tb05.45E22.810 Protein, major vault protein Protein, major vault protein L homeolog Protein, putative major vault protein Protein, MVP Protein, Mvp Protein, mvp Protein, mvp.L Protein, Tc00.1047053510353.10 Protein, Tb927.5.4460 Protein, Tb10.70.0520 Protein, Tb10.70.5840 Protein, LMJF_05_0060 Protein
- 背景
- Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases. {ECO:0000269|PubMed:15133037, ECO:0000269|PubMed:16418217, ECO:0000269|PubMed:16441665}.
- 分子量
- 100.1 kDa Including tag.
- UniProt
- Q14764
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