LOXL2 Protein (AA 26-774) (His tag)
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- 抗原 See all LOXL2 蛋白
- LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 26-774
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宿主
- 人
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资源
- Insect Cells
- 标记
- This LOXL2 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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QYDSWPHYPE YFQQPAPEYH QPQAPANVAK IQLRLAGQKR KHSEGRVEVY YDGQWGTVCD DDFSIHAAHV VCRELGYVEA KSWTASSSYG KGEGPIWLDN LHCTGNEATL AACTSNGWGV TDCKHTEDVG VVCSDKRIPG FKFDNSLINQ IENLNIQVED IRIRAILSTY RKRTPVMEGY VEVKEGKTWK QICDKHWTAK NSRVVCGMFG FPGERTYNTK VYKMFASRRK QRYWPFSMDC TGTEAHISSC KLGPQVSLDP MKNVTCENGL PAVVSCVPGQ VFSPDGPSRF RKAYKPEQPL VRLRGGAYIG EGRVEVLKNG EWGTVCDDKW DLVSASVVCR ELGFGSAKEA VTGSRLGQGI GPIHLNEIQC TGNEKSIIDC KFNAESQGCN HEEDAGVRCN TPAMGLQKKL RLNGGRNPYE GRVEVLVERN GSLVWGMVCG QNWGIVEAMV VCRQLGLGFA SNAFQETWYW HGDVNSNKVV MSGVKCSGTE LSLAHCRHDG EDVACPQGGV QYGAGVACSE TAPDLVLNAE MVQQTTYLED RPMFMLQCAM EENCLSASAA QTDPTTGYRR LLRFSSQIHN NGQSDFRPKN GRHAWIWHDC HRHYHSMEVF THYDLLNLNG TKVAEGHKAS FCLEDTECEG DIQKNYECAN FGDQGITMGC WDMYRHDIDC QWVDITDVPP GDYLFQVVIN PNFEVAESDY SNNIMKCRSR YDGHRIWMYN CHIGGSFSEE TEKKFEHFSG LLNNQLSPQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human LOXL2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product LOXL2 蛋白
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Lysyl Oxidase-Like 2 (LOXL2) (AA 675-773) protein (GST tag)
LOXL2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lysyl Oxidase-Like 2 (LOXL2) (AA 26-776) protein (His tag)Crystallography grade LOXL2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lysyl Oxidase-Like 2 (LOXL2) (AA 26-776) protein (His tag)LOXL2 宿主: 小鼠 宿主: 酵母菌 Recombinant > 90 % SDS
Lysyl Oxidase-Like 2 (LOXL2) (AA 26-776) protein (His tag)LOXL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))
- 别名
- LOXL2 (LOXL2 产品)
- 别名
- LOR2 Protein, WS9-14 Protein, CG4402 Protein, Dmel\\CG4402 Protein, Dmloxl-2 Protein, dmlox-2 Protein, 1110004B06Rik Protein, 4930526G11Rik Protein, 9430067E15Rik Protein, GB13360 Protein, lox2-like Protein, LOXL2 Protein, lor2 Protein, loxl-2 Protein, ws9-14 Protein, im:7136137 Protein, si:dkeyp-32b1.1 Protein, wu:fk12g04 Protein, zgc:158414 Protein, lysyl oxidase like 2 Protein, lysyl oxidase-like 2 Protein, lysyl oxidase homolog 4 Protein, lysyl oxidase like 2 L homeolog Protein, lysyl oxidase-like 2b Protein, LOXL2 Protein, lox2 Protein, Loxl2 Protein, LOC408544 Protein, loxl2.L Protein, loxl2 Protein, loxl2b Protein
- 背景
- Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. {ECO:0000269|PubMed:16096638, ECO:0000269|PubMed:20026874, ECO:0000269|PubMed:21233336, ECO:0000269|PubMed:21732535, ECO:0000269|PubMed:21835952, ECO:0000269|PubMed:22483618}.
- 分子量
- 85.0 kDa Including tag.
- UniProt
- Q9Y4K0
- 途径
- Chromatin Binding
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