HSP90AA1 Protein (AA 2-732) (His tag)
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- 抗原 See all HSP90AA1 蛋白
- HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-732
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宿主
- 人
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资源
- Insect Cells
- 标记
- This HSP90AA1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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PEETQTQDQP MEEEEVETFA FQAEIAQLMS LIINTFYSNK EIFLRELISN SSDALDKIRY ESLTDPSKLD SGKELHINLI PNKQDRTLTI VDTGIGMTKA DLINNLGTIA KSGTKAFMEA LQAGADISMI GQFGVGFYSA YLVAEKVTVI TKHNDDEQYA WESSAGGSFT VRTDTGEPMG RGTKVILHLK EDQTEYLEER RIKEIVKKHS QFIGYPITLF VEKERDKEVS DDEAEEKEDK EEEKEKEEKE SEDKPEIEDV GSDEEEEKKD GDKKKKKKIK EKYIDQEELN KTKPIWTRNP DDITNEEYGE FYKSLTNDWE DHLAVKHFSV EGQLEFRALL FVPRRAPFDL FENRKKKNNI KLYVRRVFIM DNCEELIPEY LNFIRGVVDS EDLPLNISRE MLQQSKILKV IRKNLVKKCL ELFTELAEDK ENYKKFYEQF SKNIKLGIHE DSQNRKKLSE LLRYYTSASG DEMVSLKDYC TRMKENQKHI YYITGETKDQ VANSAFVERL RKHGLEVIYM IEPIDEYCVQ QLKEFEGKTL VSVTKEGLEL PEDEEEKKKQ EEKKTKFENL CKIMKDILEK KVEKVVVSNR LVTSPCCIVT STYGWTANME RIMKAQALRD NSTMGYMAAK KHLEINPDHS IIETLRQKAE ADKNDKSVKD LVILLYETAL LSSGFSLEDP QTHANRIYRM IKLGLGIDED DPTADDTSAA VTEEMPPLEG DDDTSRMEEV D
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human HSP90AA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product HSP90AA1 蛋白
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Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (AA 381-732) protein (His-IF2DI Tag)
HSP90AA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (AA 1-732) protein (His tag)HSP90AA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (AA 2-733) protein (His tag)Crystallography grade HSP90AA1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (AA 535-732) protein (GST tag)HSP90AA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >85 % as determined by SDS-PAGE.
Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)HSP90AA1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)HSP90AA1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
- 别名
- HSP90AA1 (HSP90AA1 产品)
- 别名
- EL52 Protein, HSP86 Protein, HSP89A Protein, HSP90A Protein, HSP90N Protein, HSPC1 Protein, HSPCA Protein, HSPCAL1 Protein, HSPCAL4 Protein, HSPN Protein, Hsp89 Protein, Hsp90 Protein, LAP2 Protein, Hsp86 Protein, Hspca Protein, htpG Protein, 86kDa Protein, 89kDa Protein, AL024080 Protein, AL024147 Protein, Hsp86-1 Protein, hsp4 Protein, HSP90 Protein, HSP90AA1 Protein, fb17b01 Protein, hsp90 Protein, hsp90a Protein, hsp90a.1 Protein, hsp90alpha Protein, wu:fb17b01 Protein, zgc:86652 Protein, Hsp90alpha Protein, heat shock protein 90 alpha family class A member 1 Protein, heat shock protein 90, alpha (cytosolic), class A member 1 Protein, Heat Shock Protein 90, cytosolic Protein, heat shock protein 90A Protein, molecular chaperone Protein, heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1 Protein, heat shock protein HSP 90-alpha Protein, heat shock protein 90kDa alpha (cytosolic), class A member 1 Protein, HSP90AA1 Protein, Hsp90aa1 Protein, HSP90A Protein, hsp90A Protein, hsp90aa1.1 Protein, LOC108698781 Protein
- 背景
- Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. {ECO:0000269|PubMed:11274138, ECO:0000269|PubMed:11276205, ECO:0000269|PubMed:15577939, ECO:0000269|PubMed:15937123}.
- 分子量
- 85.5 kDa Including tag.
- UniProt
- P07900
- 途径
- M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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