GRIK1 Protein (AA 31-576) (His tag)
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- 抗原 See all GRIK1 蛋白
- GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 31-576
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宿主
- 人
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资源
- Insect Cells
- 标记
- This GRIK1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
- 序列
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QTAPQVLRIG GIFETVENEP VNVEELAFKF AVTSINRNRT LMPNTTLTYD IQRINLFDSF EASRRACDQL ALGVAALFGP SHSSSVSAVQ SICNALEVPH IQTRWKHPSV DNKDLFYINL YPDYAAISRA ILDLVLYYNW KTVTVVYEDS TGLIRLQELI KAPSRYNIKI KIRQLPSGNK DAKPLLKEMK KGKEFYVIFD CSHETAAEIL KQILFMGMMT EYYHYFFTTL DLFALDLELY RYSGVNMTGF RLLNIDNPHV SSIIEKWSME RLQAPPRPET GLLDGMMTTE AALMYDAVYM VAIASHRASQ LTVSSLQCHR HKPWRLGPRF MNLIKEARWD GLTGHITFNK TNGLRKDFDL DIISLKEEGT EKAAGEVSKH LYKVWKKIGI WNSNSGLNMT DSNKDKSSNI TDSLANRTLI VTTILEEPYV MYRKSDKPLY GNDRFEGYCL DLLKELSNIL GFIYDVKLVP DGKYGAQNDK GEWNGMVKEL IDHRADLAVA PLTITYVREK VIDFSKPFMT LGISILYRKP NGTNPGVFSF LNPLSP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human GRIK1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product GRIK1 蛋白
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Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1) (AA 331-440) protein (GST tag)
GRIK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1) (AA 675-834) protein (His tag)Crystallography grade GRIK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1) (AA 31-561) protein (His tag)Crystallography grade GRIK1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1) (AA 31-918) protein (rho-1D4 tag)Crystallography grade GRIK1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1) (AA 31-836) protein (rho-1D4 tag)Crystallography grade GRIK1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))
- 别名
- GRIK1 (GRIK1 产品)
- 别名
- GRIK1 Protein, grik1 Protein, fv36b03 Protein, wu:fv36b03 Protein, EAA3 Protein, EEA3 Protein, GLR5 Protein, GLUR5 Protein, GluK1 Protein, A830007B11Rik Protein, D16Ium24 Protein, D16Ium24e Protein, GluK5 Protein, Glur-5 Protein, Glur5 Protein, Glurbeta1 Protein, GluR5 Protein, glutamate ionotropic receptor kainate type subunit 1 Protein, glutamate receptor, ionotropic, kainate 1a Protein, glutamate receptor, ionotropic, kainate 1 Protein, GRIK1 Protein, grik1a Protein, Grik1 Protein
- 背景
- Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus.
- 分子量
- 63.1 kDa Including tag.
- UniProt
- P39086
- 途径
- Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity
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