ERAP1 Protein (AA 22-941) (His tag)
-
- 抗原 See all ERAP1 蛋白
- ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-941
-
宿主
- 人
-
资源
- Insect Cells
- 标记
- This ERAP1 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
-
ALLTVSTPSW CQSTEASPKR SDGTPFPWNK IRLPEYVIPV HYDLLIHANL TTLTFWGTTK VEITASQPTS TIILHSHHLQ ISRATLRKGA GERLSEEPLQ VLEHPRQEQI ALLAPEPLLV GLPYTVVIHY AGNLSETFHG FYKSTYRTKE GELRILASTQ FEPTAARMAF PCFDEPAFKA SFSIKIRREP RHLAISNMPL VKSVTVAEGL IEDHFDVTVK MSTYLVAFII SDFESVSKIT KSGVKVSVYA VPDKINQADY ALDAAVTLLE FYEDYFSIPY PLPKQDLAAI PDFQSGAMEN WGLTTYRESA LLFDAEKSSA SSKLGITMTV AHELAHQWFG NLVTMEWWND LWLNEGFAKF MEFVSVSVTH PELKVGDYFF GKCFDAMEVD ALNSSHPVST PVENPAQIRE MFDDVSYDKG ACILNMLREY LSADAFKSGI VQYLQKHSYK NTKNEDLWDS MASICPTDGV KGMDGFCSRS QHSSSSSHWH QEGVDVKTMM NTWTLQKGFP LITITVRGRN VHMKQEHYMK GSDGAPDTGY LWHVPLTFIT SKSDMVHRFL LKTKTDVLIL PEEVEWIKFN VGMNGYYIVH YEDDGWDSLT GLLKGTHTAV SSNDRASLIN NAFQLVSIGK LSIEKALDLS LYLKHETEIM PVFQGLNELI PMYKLMEKRD MNEVETQFKA FLIRLLRDLI DKQTWTDEGS VSERMLRSQL LLLACVHNYQ PCVQRAEGYF RKWKESNGNL SLPVDVTLAV FAVGAQSTEG WDFLYSKYQF SLSSTEKSQI EFALCRTQNK EKLQWLLDES FKGDKIKTQE FPQILTLIGR NPVGYPLAWQ FLRKNWNKLV QKFELGSSSI AHMVMGTTNQ FSTRTRLEEV KGFFSSLKEN GSQLRCVQQT IETIEENIGW MDKNFDKIRV WLQSEKLERM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human ERAP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ERAP1 蛋白
-
Endoplasmic Reticulum Aminopeptidase 1 (ERAP1) (AA 832-941) protein (GST tag)
ERAP1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Endoplasmic Reticulum Aminopeptidase 1 (ERAP1) (AA 1-941) protein (Strep Tag)Crystallography grade ERAP1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Endoplasmic Reticulum Aminopeptidase 1 (ERAP1) (AA 1-930) protein (Strep Tag)Crystallography grade ERAP1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Endoplasmic Reticulum Aminopeptidase 1 (ERAP1) (AA 24-930) protein (His tag)Crystallography grade ERAP1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Endoplasmic Reticulum Aminopeptidase 1 (ERAP1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ERAP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Endoplasmic Reticulum Aminopeptidase 1 (ERAP1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ERAP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))
- 别名
- ERAP1 (ERAP1 产品)
- 别名
- MGC166160 Protein, DKFZp469C044 Protein, ARTS-1 Protein, A-LAP Protein, ALAP Protein, APPILS Protein, ARTS1 Protein, ERAAP Protein, ERAAP1 Protein, PILS-AP Protein, PILSAP Protein, Arts1 Protein, PILSA Protein, Appils Protein, endoplasmic reticulum aminopeptidase 1 Protein, endoplasmic reticulum aminopeptidase 1b Protein, ERAP1 Protein, erap1 Protein, erap1b Protein, Erap1 Protein
- 背景
- Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. {ECO:0000269|PubMed:15908954, ECO:0000269|PubMed:16286653, ECO:0000269|PubMed:21478864}.
- 分子量
- 105.8 kDa Including tag.
- UniProt
- Q9NZ08
-
