EPB41L3 Protein (AA 2-1087) (His tag)
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- 抗原 See all EPB41L3 蛋白
- EPB41L3 (Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-1087
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宿主
- 人
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资源
- Insect Cells
- 标记
- This EPB41L3 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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TTESGSDSES KPDQEAEPQE AAGAQGRAGA PVPEPPKEEQ QQALEQFAAA AAHSTPVRRE VTDKEQEFAA RAAKQLEYQQ LEDDKLSQKS SSSKLSRSPL KIVKKPKSMQ CKVILLDGSE YTCDVEKRSR GQVLFDKVCE HLNLLEKDYF GLTYRDAENQ KNWLDPAKEI KKQVRSGAWH FSFNVKFYPP DPAQLSEDIT RYYLCLQLRD DIVSGRLPCS FVTLALLGSY TVQSELGDYD PDECGSDYIS EFRFAPNHTK ELEDKVIELH KSHRGMTPAE AEMHFLENAK KLSMYGVDLH HAKDSEGVEI MLGVCASGLL IYRDRLRINR FAWPKVLKIS YKRNNFYIKI RPGEFEQFES TIGFKLPNHR AAKRLWKVCV EHHTFFRLLL PEAPPKKFLT LGSKFRYSGR TQAQTRRASA LIDRPAPYFE RSSSKRYTMS RSLDGEVGTG QYATTKGISQ TNLITTVTPE KKAEEERDEE EDKRRKGEEV TPISAIRHEG KSPGLGTDSC PLSPPSTHCA PTSPTELRRR CKENDCKLPG YEPSRAEHLP GEPALDSDGP GRPYLGDQDV AFSYRQQTGK GTTLFSFSLQ LPESFPSLLD DDGYLSFPNL SETNLLPQSL QHYLPIRSPS LVPCFLFIFF FLLSASFSVP YALTLSFPLA LCLCYLEPKA ASLSASLDND PSDSSEEETD SERTDTAADG ETTATESDQE EDAELKAQEL EKTQDDLMKH QTNISELKRT FLETSTDTAV TNEWEKRLST SPVRLAARQE DAPMIEPLVP EETKQSSGEK LMDGSEIFSL LESARKPTEF IGGVTSTSQS WVQKMETKTE SSGIETEPTV HHLPLSTEKV VQETVLVEER RVVHASGDAS YSAGDSGDAA AQPAFTGIKG KEGSALTEGA KEEGGEEVAK AVLEQEETAA ASRERQEEQS AAIHISETLE QKPHFESSTV KTETISFGSV SPGGVKLEIS TKEVPVVHTE TKTITYESSQ VDPGTDLEPG VLMSAQTITS ETTSTTTTTH ITKTVKGGIS ETRIEKRIVI TGDADIDHDQ ALAQAIKEAK EQHPDMSVTK VVVHKETEIT PEDGED
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human EPB41L3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EPB41L3 蛋白
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Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 3-194) protein (His tag)
EPB41L3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 1-865) protein (GST tag)EPB41L3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 915-1007) protein (GST tag)EPB41L3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 1-1087) protein (Strep Tag)Crystallography grade EPB41L3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 1-929) protein (Strep Tag)Crystallography grade EPB41L3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 2-929) protein (His tag)Crystallography grade EPB41L3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EPB41L3 (Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3))
- 别名
- EPB41L3 (EPB41L3 产品)
- 别名
- 4.1B Protein, DAL-1 Protein, DAL1 Protein, DAL1P Protein, Dal1 Protein, Epb41l3 Protein, NBL3 Protein, Epb4.1l3 Protein, Kiaa0987 Protein, EPB4.1L3 Protein, dal-1 Protein, dal1 Protein, epb41l3 Protein, cb455 Protein, prcb4.1 Protein, zgc:85963 Protein, zgc:92004 Protein, erythrocyte membrane protein band 4.1 like 3 Protein, erythrocyte membrane protein band 4.1-like 3 Protein, erythrocyte membrane protein band 4.1-like 3 L homeolog Protein, erythrocyte membrane protein band 4.1-like 3b Protein, erythrocyte membrane protein band 4.1-like 3a Protein, EPB41L3 Protein, Epb41l3 Protein, epb41l3.L Protein, epb41l3b Protein, epb41l3a Protein
- 背景
- Tumor suppressor that inhibits cell proliferation and promotes apoptosis. Modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5. {ECO:0000269|PubMed:15334060, ECO:0000269|PubMed:15737618, ECO:0000269|PubMed:16420693, ECO:0000269|PubMed:9892180}.
- 分子量
- 121.5 kDa Including tag.
- UniProt
- Q9Y2J2
- 途径
- Cell-Cell Junction Organization, Maintenance of Protein Location
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