COMMD1 Protein (AA 2-190) (His tag)
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- 抗原 See all COMMD1 蛋白
- COMMD1 (Copper Metabolism (Murr1) Domain Containing 1 (COMMD1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-190
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This COMMD1 protein is labelled with His tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- 序列
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AAGELEGGKP LSGLLNALAQ DTFHGYPGIT EELLRSQLYP EVPPEEFRPF LAKMRGILKS IASADMDFNQ LEAFLTAQTK KQGGITSDQA AVISKFWKSH KTKIRESLMN QSRWNSGLRG LSWRVDGKSQ SRHSAQIHTP VAIIELELGK YGQESEFLCL EFDEVKVNQI LKTLSEVEES ISTLISQPN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human COMMD1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product COMMD1 蛋白
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Copper Metabolism (Murr1) Domain Containing 1 (COMMD1) (AA 1-190) protein (His tag)
COMMD1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Copper Metabolism (Murr1) Domain Containing 1 (COMMD1) (AA 1-190) protein (GST tag)COMMD1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Copper Metabolism (Murr1) Domain Containing 1 (COMMD1) (AA 91-190) protein (GST tag)COMMD1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Copper Metabolism (Murr1) Domain Containing 1 (COMMD1) (AA 1-188) protein (Strep Tag)Crystallography grade COMMD1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Copper Metabolism (Murr1) Domain Containing 1 (COMMD1) protein (Myc-DYKDDDDK Tag)COMMD1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- COMMD1 (Copper Metabolism (Murr1) Domain Containing 1 (COMMD1))
- 别名
- COMMD1 (COMMD1 产品)
- 别名
- C2orf5 Protein, MURR1 Protein, murr1 Protein, commd1 Protein, si:ch211-213b8.2 Protein, DDBDRAFT_0184206 Protein, DDBDRAFT_0266452 Protein, DDB_0184206 Protein, DDB_0266452 Protein, COMMD1 Protein, MGC114682 Protein, AI256843 Protein, Murr1 Protein, U2/Mu Protein, copper metabolism domain containing 1 Protein, copper metabolism (Murr1) domain containing 1 Protein, COMM domain-containing protein 1 Protein, copper metabolism domain containing 1 L homeolog Protein, COMM domain containing 1 Protein, COMMD1 Protein, commd1 Protein, comd1 Protein, Commd1 Protein, commd1.L Protein
- 背景
- Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1, in vitro promotes CRL E3 activity and dissociates CAND1 from CUL1 and CUL2 (PubMed:21778237). Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity (PubMed:15799966, PubMed:17183367, PubMed:20048074). Involved in the regulation of membrane expression and ubiquitination of SLC12A2 (PubMed:23515529). Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes (PubMed:14645214, PubMed:20237237, PubMed:21741370). Promotes CFTR cell surface expression through regulation of its ubiquitination (PubMed:21483833). Down-regulates SOD1 activity by interfering with its homodimerization (PubMed:20595380). Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery, the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed:25355947). Can bind one copper ion per monomer (PubMed:17309234). May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:18940794). Involved in the regulation of HIF1A-mediated transcription, competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1 (PubMed:20458141). {ECO:0000269|PubMed:14645214, ECO:0000269|PubMed:14685266, ECO:0000269|PubMed:15799966, ECO:0000269|PubMed:16573520, ECO:0000269|PubMed:17183367, ECO:0000269|PubMed:17309234, ECO:0000269|PubMed:20048074, ECO:0000269|PubMed:20237237, ECO:0000269|PubMed:20458141, ECO:0000269|PubMed:20595380, ECO:0000269|PubMed:21483833, ECO:0000269|PubMed:21741370, ECO:0000269|PubMed:21778237, ECO:0000269|PubMed:23515529, ECO:0000269|PubMed:25355947}.
- 分子量
- 22.0 kDa Including tag.
- UniProt
- Q8N668
- 途径
- Transition Metal Ion Homeostasis
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