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CEP170 Protein (AA 1-1584) (Strep Tag)

Crystallography grade CEP170 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
产品编号 ABIN3091613
发货至: 中国
  • 抗原 See all CEP170 products
    CEP170 (Centrosomal Protein 170kDa (CEP170))
    蛋白类型
    Recombinant
    产品特性
    AA 1-1584
    宿主
    资源
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This CEP170 protein is labelled with Strep Tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    序列
    MSLTSWFLVS SGGTRHRLPR EMIFVGRDDC ELMLQSRSVD KQHAVINYDA STDEHLVKDL GSLNGTFVND VRIPEQTYIT LKLEDKLRFG YDTNLFTVVQ GEMRVPEEAL KHEKFTIQLQ LSQKSSESEL SKSASAKSID SKVADAATEV QHKTTEALKS EEKAMDISAM PRGTPLYGQP SWWGDDEVDE KRAFKTNGKP EEKNHEAGTS GCGIDAKQVE EQSAAANEEV LFPFCREPSY FEIPTKEFQQ PSQITESTIH EIPTKDTPSS HITGAGHASF TIEFDDSTPG KVTIRDHVTK FTSDQRHKSK KSSPGTQDLL GIQTGMMAPE NKVADWLAQN NPPQMLWERT EEDSKSIKSD VPVYLKRLKG NKHDDGTQSD SENAGAHRRC SKRATLEEHL RRHHSEHKKL QKVQATEKHQ DQAVTSSAHH RGGHGVPHGK LLKQKSEEPS VSIPFLQTAL LRSSGSLGHR PSQEMDKMLK NQATSATSEK DNDDDQSDKG TYTIELENPN SEEVEARKMI DKVFGVDDNQ DYNRPVINEK HKDLIKDWAL SSAAAVMEER KPLTTSGFHH SEEGTSSSGS KRWVSQWASL AANHTRHDQE ERIMEFSAPL PLENETEISE SGMTVRSTGS ATSLASQGER RRRTLPQLPN EEKSLESHRA KVVTQRSEIG EKQDTELQEK ETPTQVYQKD KQDADRPLSK MNRAVNGETL KTGGDNKTLL HLGSSAPGKE KSETDKETSL VKQTLAKLQQ QEQREEAQWT PTKLSSKNVS GQTDKCREET FKQESQPPEK NSGHSTSKGD RVAQSESKRR KAEEILKSQT PKGGDKKESS KSLVRQGSFT IEKPSPNIPI ELIPHINKQT SSTPSSLALT SASRIRERSE SLDPDSSMDT TLILKDTEAV MAFLEAKLRE DNKTDEGPDT PSYNRDNSIS PESDVDTAST ISLVTGETER KSTQKRKSFT SLYKDRCSTG SPSKDVTKSS SSGAREKMEK KTKSRSTDVG SRADGRKFVQ SSGRIRQPSV DLTDDDQTSS VPHSAISDIM SSDQETYSCK PHGRTPLTSA DEHVHSKLEG SKVTKSKTSP VVSGSSSKST TLPRPRPTRT SLLRRARLGE ASDSELADAD KASVASEVST TSSTSKPPTG RRNISRIDLL AQPRRTRLGS LSARSDSEAT ISRSSASSRT AEAIIRSGAR LVPSDKFSPR IRANSISRLS DSKVKSMTSA HGSASVNSRW RRFPTDYAST SEDEFGSNRN SPKHTRLRTS PALKTTRLQS AGSAMPTSSS FKHRIKEQED YIRDWTAHRE EIARISQDLA LIAREINDVA GEIDSVTSSG TAPSTTVSTA ATTPGSAIDT REELVDRVFD ESLNFRKIPP LVHSKTPEGN NGRSGDPRPQ AAEPPDHLTI TRRRTWSRDE VMGDNLLLSS VFQFSKKIRQ SIDKTAGKIR ILFKDKDRNW DDIESKLRAE SEVPIVKTSS MEISSILQEL KRVEKQLQAI NAMIDPDGTL EALNNMGFPS AMLPSPPKQK SSPVNNHHSP GQTPTLGQPE ARALHPAAVS AAAEFENAES EADFSIHFNR FNPDGEEEDV TVQE
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    CEP170 (Centrosomal Protein 170kDa (CEP170))
    别名
    CEP170 (CEP170 产品)
    别名
    KAB1 Protein, Kab Protein, Rhk1 Protein, CEP170 Protein, MGC127959 Protein, 4933426L22Rik Protein, A330004A13Rik Protein, AI195353 Protein, FAM68A Protein, KAB Protein, KIAA0470 Protein, centrosomal protein 170 Protein, Cep170 Protein, CEP170 Protein, cep170 Protein
    背景
    Centrosomal protein of 170 kDa (Cep170) (KARP-1-binding protein) (KARP1-binding protein),FUNCTION: Plays a role in microtubule organization (PubMed:15616186). Required for centriole subdistal appendage assembly (PubMed:28422092). {ECO:0000269|PubMed:15616186, ECO:0000269|PubMed:28422092}.
    分子量
    175.3 kDa
    UniProt
    Q5SW79
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