CHMP3 Protein (AA 2-222) (His tag)
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- 抗原 See all CHMP3 蛋白
- CHMP3 (Charged Multivesicular Body Protein 3 (CHMP3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-222
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CHMP3 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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GLFGKTQEKP PKELVNEWSL KIRKEMRVVD RQIRDIQREE EKVKRSVKDA AKKGQKDVCI VLAKEMIRSR KAVSKLYASK AHMNSVLMGM KNQLAVLRVA GSLQKSTEVM KAMQSLVKIP EIQATMRELS KEMMKAGIIE EMLEDTFESM DDQEEMEEEA EMEIDRILFE ITAGALGKAP SKVTDALPEP EPPGAMAASE DEEEEEEALE AMQSRLATLR S
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CHMP3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product CHMP3 蛋白
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Charged Multivesicular Body Protein 3 (CHMP3) (AA 1-222) protein (His tag)
CHMP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Charged Multivesicular Body Protein 3 (CHMP3) (AA 1-222) protein (GST tag)CHMP3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Charged Multivesicular Body Protein 3 (CHMP3) (AA 2-224) protein (His tag)Crystallography grade CHMP3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Charged Multivesicular Body Protein 3 (CHMP3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CHMP3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- CHMP3 (Charged Multivesicular Body Protein 3 (CHMP3))
- 别名
- CHMP3 (CHMP3 产品)
- 别名
- CG9779 Protein, Dmel\\CG9779 Protein, Vps24 Protein, dvps24 Protein, vps24 Protein, VPS24 Protein, CHMP3 Protein, GB15107 Protein, wu:fb47g01 Protein, zgc:76972 Protein, NEDF Protein, 25.1 Protein, 4921505F14Rik Protein, 9130011K15Rik Protein, CGI-49 Protein, D6Ertd286e Protein, Vacuolar protein sorting 24 Protein, charged multivesicular body protein 3 Protein, SNF7 family protein Protein, hypothetical protein Protein, neuroendocrine differentiation factor, putative Protein, charged multivesicular body protein 3 S homeolog Protein, Vps24 Protein, chmp3 Protein, VPS24.2 Protein, CpipJ_CPIJ004098 Protein, PGTG_02107 Protein, LOC5569267 Protein, Smp_032150 Protein, CHMP3 Protein, LOC410607 Protein, Chmp3 Protein, chmp3.S Protein
- 背景
- Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress-mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells. {ECO:0000269|PubMed:14505570, ECO:0000269|PubMed:15707591, ECO:0000269|PubMed:16740483, ECO:0000269|PubMed:17331679, ECO:0000269|PubMed:18076377}.
- 分子量
- 25.9 kDa Including tag.
- UniProt
- Q9Y3E7
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