HSPE1 Protein (AA 2-102) (His tag)
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- 抗原 See all HSPE1 蛋白
- HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-102
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This HSPE1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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AGQAFRKFLP LFDRVLVERS AAETVTKGGI MLPEKSQGKV LQATVVAVGS GSKGKGGEIQ PVSVKVGDKV LLPEYGGTKV VLDDKDYFLF RDGDILGKYV D
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human HSPE1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product HSPE1 蛋白
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Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) (AA 1-102) protein (GST tag)
HSPE1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) proteinHSPE1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90% WB, SDS
Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) (AA 2-102) protein (His-Avi Tag)HSPE1 宿主: 人, 小鼠, 山羊 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) (AA 1-102) protein (His tag)HSPE1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) (AA 1-102) protein (His-IF2DI Tag)HSPE1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) (AA 2-102) protein (His-Avi Tag,Biotin)HSPE1 宿主: 人, 小鼠, 山羊 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) (AA 2-102), (full length) protein (His tag)HSPE1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) protein (Myc-DYKDDDDK Tag)HSPE1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))
- 别名
- HSPE1 (HSPE1 产品)
- 别名
- CPN10 Protein, EPF Protein, GROES Protein, HSP10 Protein, groS Protein, Cpn10 Protein, cpn10 Protein, zgc:86747 Protein, hspe1 Protein, MGC89647 Protein, HSPE1 Protein, MITOCHONDRIAL CHAPERONIN 10 Protein, T15D22.2 Protein, T15D22_2 Protein, chaperonin 10 Protein, Dmel\\CG11267 Protein, Hsp10 Protein, anon-WO0118547.175 Protein, hsp10 Protein, crpB Protein, chpS Protein, 10kDa Protein, mt-cpn10 Protein, heat shock protein family E (Hsp10) member 1 Protein, molecular chaperone GroES Protein, chaperonin 10 kDa Protein, chaperonin, 10 kDa Protein, chaperonin-10 kDa Protein, heat shock protein family E member 1 Protein, heat shock 10 protein 1 Protein, heat shock protein family E (Hsp10) member 1 L homeolog Protein, heat shock 10kDa protein 1 (chaperonin 10) pseudogene Protein, 10 kd chaperonin Protein, chaperonin 10 Protein, Hsp10p Protein, co-chaperonin 10, mitochondrial Protein, CG11267 gene product from transcript CG11267-RA Protein, co-chaperonin GroES Protein, mitochondrial heat shock protein Hsp10 (predicted) Protein, chaperonin GroS Protein, Hsp10 10 kDa chaperonin GROES Protein, mitochondrial heat shock protein Hsp10 Protein, heat shock protein 10 Protein, heat shock protein 1 (chaperonin 10) Protein, heat shock 10kDa protein 1 (chaperonin 10) Protein, HSPE1 Protein, groES Protein, groES2 Protein, TP01_0190 Protein, Cag_1167 Protein, Bm1_56470 Protein, Hspe1 Protein, hspe1 Protein, hspe1.L Protein, LOC452179 Protein, Cpn10 Protein, CPN10 Protein, HSP10 Protein, CG11267 Protein, hsp10 Protein, groS Protein
- 背景
- Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
- 分子量
- 11.8 kDa Including tag.
- UniProt
- P61604
- 途径
- Positive Regulation of Endopeptidase Activity
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