Elastase 3B Protein (AA 29-270) (His tag)
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- 抗原 See all Elastase 3B (ELA3B) 蛋白
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 29-270
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宿主
- 人
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资源
- Insect Cells
- 标记
- This Elastase 3B protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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VVNGEDAVPY SWPWQVSLQY EKSGSFYHTC GGSLIAPDWV VTAGHCISSS RTYQVVLGEY DRAVKEGPEQ VIPINSGDLF VHPLWNRSCV ACGNDIALIK LSRSAQLGDA VQLASLPPAG DILPNETPCY ITGWGRLYTN GPLPDKLQEA LLPVVDYEHC SRWNWWGSSV KKTMVCAGGD IRSGCNGDSG GPLNCPTEDG GWQVHGVTSF VSAFGCNTRR KPTVFTRVSA FIDWIEETIA SH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CELA3B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ELA3B 蛋白
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Elastase 3B, Pancreatic (ELA3B) (AA 28-269) protein (His tag)
ELA3B 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Elastase 3B, Pancreatic (ELA3B) (AA 19-270) protein (GST tag)ELA3B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Elastase 3B, Pancreatic (ELA3B) (AA 1-270) protein (GST tag)ELA3B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Elastase 3B, Pancreatic (ELA3B) (AA 28-269) protein (His tag)Crystallography grade ELA3B 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Elastase 3B, Pancreatic (ELA3B) proteinELA3B 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
Elastase 3B, Pancreatic (ELA3B) proteinELA3B 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
- 别名
- CELA3B (ELA3B 产品)
- 别名
- CBPP Protein, E1 Protein, EL-1 Protein, ELA3B Protein, 0910001F22Rik Protein, 2310074F01Rik Protein, AI504000 Protein, Ela3 Protein, Ela3b Protein, ela3b Protein, MGC82867 Protein, ELA1 Protein, Cela3b Protein, CELA3B Protein, chymotrypsin like elastase family member 3B Protein, chymotrypsin-like elastase family, member 3B Protein, elastase 3B, pancreatic Protein, proproteinase E Protein, chymotrypsin like elastase family member 3B L homeolog Protein, chymotrypsin-like elastase family member 3B Protein, CELA3B Protein, Cela3b Protein, ela3b Protein, LOC478196 Protein, cela3b.L Protein, LOC100717936 Protein, LOC100071692 Protein
- 背景
- Efficient protease with alanine specificity but only little elastolytic activity.
- 分子量
- 27.3 kDa Including tag.
- UniProt
- P08861
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