CACNA1A Protein (AA 715-1242) (His tag)
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- 抗原 See all CACNA1A 蛋白
- CACNA1A (Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 715-1242
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宿主
- 人
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资源
- Insect Cells
- 标记
- This CACNA1A protein is labelled with His tag.
- 应用范围
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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DNLANAQELT KDEQEEEEAA NQKLALQKAK EVAEVSPLSA ANMSIAVKEQ QKNQKPAKSV WEQRTSEMRK QNLLASREAL YNEMDPDERW KAAYTRHLRP DMKTHLDRPL VVDPQENRNN NTNKSRAAEP TVDQRLGQQR AEDFLRKQAR YHDRARDPSG SAGLDARRPW AGSQEAELSR EGPYGRESDH HAREGSLEQP GFWEGEAERG KAGDPHRRHV HRQGGSRESR SGSPRTGADG EHRRHRAHRR PGEEGPEDKA ERRARHREGS RPARGGEGEG EGPDGGERRR RHRHGAPATY EGDARREDKE RRHRRRKENQ GSGVPVSGPN LSTTRPIQQD LGRQDPPLAE DIDNMKNNKL ATAESAAPHG SLGHAGLPQS PAKMGNSTDP GPMLAIPAMA TNPQNAASRR TPNNPGNPSN PGPPKTPENS LIVTNPSGTQ TNSAKTARKP DHTTVDIPPA CPPPLNHTVV QVNKNANPDP LPKKEEEKKE EEEDDRGEDG PKPMPPYSSM FILSTTNPLR RLCHYILN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CACNA1A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product CACNA1A 蛋白
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Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (AA 361-487) protein (His tag)
Crystallography grade CACNA1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (AA 363-489) protein (His tag)Crystallography grade CACNA1A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (AA 1808-2505) protein (His tag)Crystallography grade CACNA1A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (AA 1764-2368) protein (His tag)Crystallography grade CACNA1A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) (AA 717-1190) protein (His tag)Crystallography grade CACNA1A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- CACNA1A (Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A))
- 别名
- CACNA1A (CACNA1A 产品)
- 别名
- cav2.1 Protein, cacna-a Protein, ca(v)2.1 Protein, cacna1a Protein, si:ch211-237k17.1 Protein, BccA1 Protein, Cav2.1 Protein, rbA-1 Protein, APCA Protein, BI Protein, CACNL1A4 Protein, CAV2.1 Protein, EA2 Protein, FHM Protein, HPCA Protein, MHP Protein, MHP1 Protein, SCA6 Protein, Caca1a Protein, Cacnl1a4 Protein, Ccha1a Protein, alpha1A Protein, la Protein, leaner Protein, nmf352 Protein, rkr Protein, rocker Protein, tg Protein, tottering Protein, CACH4 Protein, CACN3 Protein, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit S homeolog Protein, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a Protein, calcium voltage-gated channel subunit alpha1 A Protein, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit Protein, P/Q-type voltage-gated calcium channel alpha1A subunit ChCaChA1A Protein, cacna1a.S Protein, cacna1aa Protein, CACNA1A Protein, Cacna1a Protein, LOC395892 Protein
- 背景
- Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by the funnel toxin (Ftx) and by the omega-agatoxin-IVA (omega-Aga-IVA). They are however insensitive to dihydropyridines (DHP), and omega-conotoxin-GVIA (omega-CTx-GVIA).
- 分子量
- 59.6 kDa Including tag.
- UniProt
- O00555
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process
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