CACNA1H Protein (AA 1016-1290) (His tag)
-
- 抗原 See all CACNA1H 蛋白
- CACNA1H (Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1016-1290
-
宿主
- 人
-
资源
- Insect Cells
- 标记
- This CACNA1H protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
-
EGFQAEGDAN RSDTDEDKTS VHFEEDFHKL RELQTTELKM CSLAVTPNGH LEGRGSLSPP LIMCTAATPM PTPKSSPFLD AAPSLPDSRR GSSSSGDPPL GDQKPPASLR SSPCAPWGPS GAWSSRRSSW SSLGRAPSLK RRGQCGERES LLSGEGKGST DDEAEDGRAA PGPRATPLRR AESLDPRPLR PAALPPTKCR DRDGQVVALP SDFFLRIDSH REDAAELDDD SEDSCCLRLH KVLEPYKPQW CRSREAWALY LFSPQNRFRV SCQKV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human CACNA1H Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
-
Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H) (AA 254-394) protein (His tag)
Crystallography grade CACNA1H 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H) (AA 420-793) protein (His tag)Crystallography grade CACNA1H 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H) (AA 1013-1301) protein (His tag)Crystallography grade CACNA1H 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H) (AA 254-394) protein (His tag)Crystallography grade CACNA1H 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H) (AA 420-790) protein (His tag)Crystallography grade CACNA1H 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H) (AA 1864-2353) protein (His tag)Crystallography grade CACNA1H 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H) (AA 1875-2365) protein (His tag)Crystallography grade CACNA1H 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- CACNA1H (Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H))
- 别名
- CACNA1H (CACNA1H 产品)
- 别名
- CACNA1HB Protein, Cav3.2 Protein, ECA6 Protein, EIG6 Protein, MNCb-1209 Protein, alpha13.2 Protein, calcium voltage-gated channel subunit alpha1 H Protein, calcium channel, voltage-dependent, T type, alpha 1H subunit Protein, CACNA1H Protein, Cacna1h Protein
- 背景
- Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
- 分子量
- 31.1 kDa Including tag.
- UniProt
- O95180
- 途径
- C21-Steroid Hormone Metabolic Process
-
