B3GAT3 Protein (AA 29-335) (His tag)
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- 抗原 See all B3GAT3 蛋白
- B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 29-335
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宿主
- 人
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资源
- Insect Cells
- 标记
- This B3GAT3 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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QPCDCLPPLR AAAEQLRQKD LRISQLQAEL RRPPPAPAQP PEPEALPTIY VVTPTYARLV QKAELVRLSQ TLSLVPRLHW LLVEDAEGPT PLVSGLLAAS GLLFTHLVVL TPKAQRLREG EPGWVHPRGV EQRNKALDWL RGRGGAVGGE KDPPPPGTQG VVYFADDDNT YSRELFEEMR WTRGVSVWPV GLVGGLRFEG PQVQDGRVVG FHTAWEPSRP FPVDMAGFAV ALPLLLDKPN AQFDSTAPRG HLESSLLSHL VDPKDLEPRA ANCTRVLVWH TRTEKPKMKQ EEQLQRQGRG SDPAIEV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human B3GAT3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product B3GAT3 蛋白
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beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 1-335) protein (GST tag)
B3GAT3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 236-335) protein (GST tag)B3GAT3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 1-335) protein (Strep Tag)Crystallography grade B3GAT3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 1-335) protein (Strep Tag)Crystallography grade B3GAT3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 29-335) protein (His tag)Crystallography grade B3GAT3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 29-335) protein (His tag)B3GAT3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) protein (Myc-DYKDDDDK Tag)B3GAT3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))
- 别名
- B3GAT3 (B3GAT3 产品)
- 别名
- GLCATI Protein, 2810405M13Rik Protein, GlcUAT-I Protein, Glcat-i Protein, beta-1,3-glucuronyltransferase 3 Protein, beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) Protein, B3GAT3 Protein, B3gat3 Protein
- 背景
- Glycosaminoglycans biosynthesis (PubMed:25893793). Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation (PubMed:24425863). {ECO:0000269|PubMed:24425863, ECO:0000269|PubMed:25893793}.
- 分子量
- 35.0 kDa Including tag.
- UniProt
- O94766
- 途径
- Glycosaminoglycan Metabolic Process
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