ARNT Protein (AA 2-789) (His tag)
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- 抗原 See all ARNT 蛋白
- ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-789
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宿主
- 人
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资源
- Insect Cells
- 标记
- This ARNT protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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AATTANPEMT SDVPSLGPAI ASGNSGPGIQ GGGAIVQRAI KRRPGLDFDD DGEGNSKFLR CDDDQMSNDK ERFARSDDEQ SSADKERLAR ENHSEIERRR RNKMTAYITE LSDMVPTCSA LARKPDKLTI LRMAVSHMKS LRGTGNTSTD GSYKPSFLTD QELKHLILEA ADGFLFIVSC ETGRVVYVSD SVTPVLNQPQ SEWFGSTLYD QVHPDDVDKL REQLSTSENA LTGRILDLKT GTVKKEGQQS SMRMCMGSRR SFICRMRCGS SSVDPVSVNR LSFVRNRCRN GLGSVKDGEP HFVVVHCTGY IKAWPPAGVS LPDDDPEAGQ GSKFCLVAIG RLQVTSSPNC TDMSNVCQPT EFISRHNIEG IFTFVDHRCV ATVGYQPQEL LGKNIVEFCH PEDQQLLRDS FQQVVKLKGQ VLSVMFRFRS KNQEWLWMRT SSFTFQNPYS DEIEYIICTN TNVKNSSQEP RPTLSNTIQR PQLGPTANLP LEMGSGQLAP RQQQQQTELD MVPGRDGLAS YNHSQVVQPV TTTGPEHSKP LEKSDGLFAQ DRDPRFSEIY HNINADQSKG ISSSTVPATQ QLFSQGNTFP PTPRPAENFR NSGLAPPVTI VQPSASAGQM LAQISRHSNP TQGATPTWTP TTRSGFSAQQ VATQATAKTR TSQFGVGSFQ TPSSFSSMSL PGAPTASPGA AAYPSLTNRG SNFAPETGQT AGQFQTRTAE GVGVWPQWQG QQPHHRSSSS EQHVQQPPAQ QPGQPEVFQE MLSMLGDQSN SYNNEEFPDL TMFPPFSE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ARNT Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ARNT 蛋白
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Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 1-789) protein (GST tag)
ARNT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 1-110) protein (GST tag)ARNT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 2-791) protein (His tag)Crystallography grade ARNT 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 1-474), (partial) protein (His tag)ARNT 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ARNT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ARNT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))
- 别名
- ARNT (ARNT 产品)
- 别名
- HIF-1-beta Protein, HIF-1beta Protein, HIF1-beta Protein, HIF1B Protein, HIF1BETA Protein, TANGO Protein, bHLHe2 Protein, D3Ertd557e Protein, Drnt Protein, ESTM42 Protein, Hif1b Protein, W08714 Protein, mKIAA4051 Protein, Arnt1 Protein, ARNT Protein, zgc:136664 Protein, arnt1 Protein, hif1b Protein, tango Protein, xArnt Protein, hif1beta Protein, hif-1beta Protein, arnt.2 Protein, MGC68805 Protein, aryl hydrocarbon receptor nuclear translocator Protein, aryl hydrocarbon receptor nuclear translocator S homeolog Protein, ARNT Protein, Arnt Protein, arnt Protein, arnt.S Protein
- 背景
- Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
- 分子量
- 87.5 kDa Including tag.
- UniProt
- P27540
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect
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