ANKLE2 Protein (AA 33-938) (His tag)
-
- 抗原 See all ANKLE2 products
- ANKLE2 (Ankyrin Repeat and LEM Domain Containing 2 (ANKLE2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 33-938
-
宿主
- 人
-
资源
- Insect Cells
- 标记
- This ANKLE2 protein is labelled with His tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- 序列
-
RRLGPRPGGL GRSGTPVPPP SAAAAPASGE MTMDALLARL KLLNPDDLRE EIVKAGLKCG PITSTTRFIF EKKLAQALLE QGGRLSSFYH HEAGVTALSQ DPQRILKPAE GNPTDQAGFS EDRDFGYSVG LNPPEEEAVT SKTCSVPPSD TDTYRAGATA SKEPPLYYGV CPVYEDVPAR NERIYVYENK KEALQAVKMI KGSRFKAFST REDAEKFARG ICDYFPSPSK TSLPLSPVKT APLFSNDRLK DGLCLSESET VNKERANSYK NPRTQDLTAK LRKAVEKGEE DTFSDLIWSN PRYLIGSGDN PTIVQEGCRY NVMHVAAKEN QASICQLTLD VLENPDFMRL MYPDDDEAML QKRIRYVVDL YLNTPDKMGY DTPLHFACKF GNADVVNVLS SHHLIVKNSR NKYDKTPEDV ICERSKNKSV ELKERIREYL KGHYYVPLLR AEETSSPVIG ELWSPDQTAE ASHVSRYGGS PRDPVLTLRA FAGPLSPAKA EDFRKLWKTP PREKAGFLHH VKKSDPERGF ERVGRELAHE LGYPWVEYWE FLGCFVDLSS QEGLQRLEEY LTQQEIGKKA QQETGEREAS CRDKATTSGS NSISVRAFLD EDDMSLEEIK NRQNAARNNS PPTVGAFGHT RCSAFPLEQE ADLIEAAEPG GPHSSRNGLC HPLNHSRTLA GKRPKAPRGE EAHLPPVSDL TVEFDKLNLQ NIGRSVSKTP DESTKTKDQI LTSRINAVER DLLEPSPADQ LGNGHRRTES EMSARIAKMS LSPSSPRHED QLEVTREPAR RLFLFGEEPS KLDQDVLAAL ECADVDPHQF PAVHRWKSAV LCYSPSDRQS WPSPAVKGRF KSQLPDLSGP HSYSPGRNSV AGSNPAKPGL GSPGRYSPVH GSQLRRMARL AELAAL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human ANKLE2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
-
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- ANKLE2 (Ankyrin Repeat and LEM Domain Containing 2 (ANKLE2))
- 别名
- ANKLE2 (ANKLE2 产品)
- 别名
- KIAA0692 Protein, LEMD7 Protein, Lem4 Protein, 1110001J12Rik Protein, AI661024 Protein, D5Ertd585e Protein, RGD1310191 Protein, ankyrin repeat and LEM domain containing 2 Protein, ANKLE2 Protein, Ankle2 Protein
- 背景
- Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. {ECO:0000269|PubMed:22770216}.
- 分子量
- 101.5 kDa Including tag.
- UniProt
- Q86XL3
-
