CHRM3 Protein (AA 253-491) (Strep Tag,His tag)
(1 reference)-
- 抗原 See all CHRM3 蛋白
- CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 253-491
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宿主
- 人
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资源
- Insect Cells
- 标记
- This CHRM3 protein is labelled with Strep Tag,His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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MHHHHHHRIY KETEKRTKEL AGLQASGTEA ETENFVHPTG SSRSCSSYEL QQQSMKRSNR RKYG RCH FWFTTKSWKP SSEQMDQDHS SSDSWNNNDA AASLENSASS DEEDIGSETR AIYSIVLKLP GHSTILNSTK LPSSDNLQVP EEELGMVDLE RKADKLQAQK SVDDGGSFPK SFSKLPIQLE SAVDTAKTSD VNSSVGKSTA TLPLSFKEAT LAKRFALKTR SQITKRKRMS LVKEKKAAQG SSGWSH PQFEK
Sequence includes N-terminal His-tag and C-terminal Strep-tag - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
- The protein is purified from the cleared cell lysate using Strep-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
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Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 1-67) protein (GST tag)
CHRM3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 1-590) protein (Strep Tag)Crystallography grade CHRM3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 1-589) protein (Strep Tag)Crystallography grade CHRM3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 252-490) protein (His tag)Crystallography grade CHRM3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 253-492) protein (His tag)CHRM3 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- In solution (20 mM Hepes, pH 7.4, 100 mM NaCl)
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).
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: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).
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- 抗原
- CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))
- 别名
- CHRM3 (CHRM3 产品)
- 别名
- EGBRS Protein, HM3 Protein, MACHR Protein, Chrm-3 Protein, M3 Protein, M3R Protein, cholinergic receptor muscarinic 3 Protein, cholinergic receptor, muscarinic 3 Protein, cholinergic receptor, muscarinic 3, cardiac Protein, CHRM3 Protein, Chrm3 Protein
- 背景
- The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. {ECO:0000269|PubMed:7565628}.
- 分子量
- 28.8 kDa including both tags
- UniProt
- P20309
- 途径
- Synaptic Membrane
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