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THEM2 Protein (AA 2-140) (His tag)

Crystallography grade ACOT13 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
产品编号 ABIN3088061
发货至: 中国
  • 抗原 See all THEM2 (ACOT13) 蛋白
    THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))
    蛋白类型
    Recombinant
    产品特性
    AA 2-140
    宿主
    • 6
    • 2
    资源
    • 4
    • 2
    • 1
    • 1
    大肠杆菌(E. Coli)
    标记
    This THEM2 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    序列
    TSMTQSLREV IKAMTKARNF ERVLGKITLV SAAPGKVICE MKVEEEHTNA IGTLHGGLTA TLVDNISTMA LLCTERGAPG VSVDMNITYM SPAKLGEDIV ITAHVLKQGK TLAFTSVDLT NKATGKLIAQ GRHTKHLGN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ACOT13 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))
    别名
    ACOT13 (ACOT13 产品)
    别名
    them2 Protein, MGC89869 Protein, si:dkey-218n20.4 Protein, THEM2 Protein, DKFZp468B074 Protein, PNAS-27 Protein, 0610006O17Rik Protein, Them2 Protein, acyl-CoA thioesterase 13 Protein, acyl-CoA thioesterase 13 S homeolog Protein, thioesterase superfamily member 2 Protein, Thioesterase superfamily protein Protein, acot13 Protein, ACOT13 Protein, acot13.S Protein, LOC733047 Protein, si605090c10 Protein, Acot13 Protein
    背景
    Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity). {ECO:0000250, ECO:0000269|PubMed:16934754, ECO:0000269|PubMed:19170545}.
    分子量
    15.8 kDa Including tag.
    UniProt
    Q9NPJ3
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