PSMD6 Protein (AA 2-389) (His tag)
-
- 抗原 See all PSMD6 蛋白
- PSMD6 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 6 (PSMD6))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-389
-
宿主
- 人
-
资源
- Insect Cells
- 标记
- This PSMD6 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
-
PLENLEEEGL PKNPDLRIAQ LRFLLSLPEH RGDAAVRDEL MAAVRDNNMA PYYEALCKSL DWQIDVDLLN KMKKANEDEL KRLDEELEDA EKNLGESEIR DAMMAKAEYL CRIGDKEGAL TAFRKTYDKT VALGHRLDIV FYLLRIGLFY MDNDLITRNT EKAKSLIEEG GDWDRRNRLK VYQGLYCVAI RDFKQAAELF LDTVSTFTSY ELMDYKTFVT YTVYVSMIAL ERPDLREKVI KGAEILEVLH SLPAVRQYLF SLYECRYSVF FQSLAVVEQE MKKDWLFAPH YRYYVREMRI HAYSQLLESY RSLTLGYMAE AFGVGVEFID QELSRFIAAG RLHCKIDKVN EIVETNRPDS KNWQYQETIK KGDLLLNRVQ KLSRVINM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human PSMD6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PSMD6 蛋白
-
Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 6 (PSMD6) (AA 292-389) protein (GST tag)
PSMD6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 6 (PSMD6) (AA 1-389) protein (GST tag)PSMD6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 6 (PSMD6) protein (Myc-DYKDDDDK Tag)PSMD6 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- PSMD6 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 6 (PSMD6))
- 别名
- PSMD6 (PSMD6 产品)
- 别名
- Rpn7 Protein, S10 Protein, SGA-113M Protein, p42A Protein, p44S10 Protein, DDBDRAFT_0190867 Protein, DDBDRAFT_0232985 Protein, DDB_0190867 Protein, DDB_0232985 Protein, 2400006A19Rik Protein, P44s10 Protein, zgc:56471 Protein, p44s10 Protein, rpn7 Protein, s10 Protein, 141231_at Protein, CG5378 Protein, Dmel\\CG5378 Protein, S10a Protein, anon-WO0172774.103 Protein, anon-WO0172774.106 Protein, proteasome 26S subunit, non-ATPase 6 Protein, 26S proteasome non-ATPase regulatory subunit 6 Protein, proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 Protein, proteasome 26S subunit, non-ATPase 6 S homeolog Protein, Regulatory particle non-ATPase 7 Protein, PSMD6 Protein, psmD6 Protein, Psmd6 Protein, psmd6 Protein, psmd6.S Protein, Rpn7 Protein, rpn-7 Protein
- 背景
- Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
- 分子量
- 46.4 kDa Including tag.
- UniProt
- Q15008
- 途径
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway
-
