MYL9 Protein (AA 2-172) (His tag)
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- 抗原 See all MYL9 蛋白
- MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-172
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This MYL9 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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SSKRAKAKTT KKRPQRATSN VFAMFDQSQI QEFKEAFNMI DQNRDGFIDK EDLHDMLASL GKNPTDEYLE GMMSEAPGPI NFTMFLTMFG EKLNGTDPED VIRNAFACFD EEASGFIHED HLRELLTTMG DRFTDEEVDE MYREAPIDKK GNFNYVEFTR ILKHGAKDKD D
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MYL9 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MYL9 蛋白
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Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9) (AA 1-118) protein (His tag)
MYL9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9) (AA 1-172) protein (His tag)MYL9 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9) (AA 1-172) protein (His tag)MYL9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9) (AA 1-118) protein (GST tag)MYL9 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9) (AA 2-172) protein (His tag)Crystallography grade MYL9 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)MYL9 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))
- 别名
- MYL9 (MYL9 产品)
- 别名
- LC20 Protein, MLC-2C Protein, MLC2 Protein, MRLC1 Protein, MYRL2 Protein, AI327049 Protein, MLC20 Protein, Mylc2c Protein, RLC-C Protein, myosin Protein, regulatory Protein, MRCL3 Protein, MRLC2 Protein, MYL9 Protein, zgc:103467 Protein, myl9 Protein, zgc:77916 Protein, myosin light chain 9 Protein, myosin, light polypeptide 9, regulatory Protein, myosin light chain 9 L homeolog Protein, myosin, light chain 9, regulatory Protein, myosin, light chain 12A, regulatory, non-sarcomeric Protein, myosin, light chain 9a, regulatory Protein, myosin, light chain 9b, regulatory Protein, MYL9 Protein, Myl9 Protein, myl9.L Protein, MYL12A Protein, myl9a Protein, myl9b Protein
- 背景
- Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
- 分子量
- 20.7 kDa Including tag.
- UniProt
- P24844
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