HNF1B Protein (AA 1-557) (Strep Tag)
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- 抗原 See all HNF1B 蛋白
- HNF1B (HNF1 Homeobox B (HNF1B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-557
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This HNF1B protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MVSKLTSLQQ ELLSALLSSG VTKEVLVQAL EELLPSPNFG VKLETLPLSP GSGAEPDTKP VFHTLTNGHA KGRLSGDEGS EDGDDYDTPP ILKELQALNT EEAAEQRAEV DRMLSEDPWR AAKMIKGYMQ QHNIPQREVV DVTGLNQSHL SQHLNKGTPM KTQKRAALYT WYVRKQREIL RQFNQTVQSS GNMTDKSSQD QLLFLFPEFS QQSHGPGQSD DACSEPTNKK MRRNRFKWGP ASQQILYQAY DRQKNPSKEE REALVEECNR AECLQRGVSP SKAHGLGSNL VTEVRVYNWF ANRRKEEAFR QKLAMDAYSS NQTHSLNPLL SHGSPHHQPS SSPPNKLSGV RYSQQGNNEI TSSSTISHHG NSAMVTSQSV LQQVSPASLD PGHNLLSPDG KMISVSGGGL PPVSTLTNIH SLSHHNPQQS QNLIMTPLSG VMAIAQSLNT SQAQSVPVIN SVAGSLAALQ PVQFSQQLHS PHQQPLMQQS PGSHMAQQPF MAAVTQLQNS HMYAHKQEPP QYSHTSRFPS AMVVTDTSSI STLTNMSSSK QCPLQAW
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product HNF1B 蛋白
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HNF1 Homeobox B (HNF1B) (AA 31-260) protein (His-IF2DI Tag)
HNF1B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
HNF1 Homeobox B (HNF1B) (AA 173-277) protein (GST tag)HNF1B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
HNF1 Homeobox B (HNF1B) (AA 29-118) protein (GST tag)HNF1B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
HNF1 Homeobox B (HNF1B) (AA 1-557) protein (GST tag)HNF1B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
HNF1 Homeobox B (HNF1B) (AA 1-558) protein (Strep Tag)Crystallography grade HNF1B 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
HNF1 Homeobox B (HNF1B) protein (Myc-DYKDDDDK Tag)HNF1B 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HNF1B (HNF1 Homeobox B (HNF1B))
- 别名
- HNF1B (HNF1B 产品)
- 别名
- FJHN Protein, HNF-1B Protein, HNF1beta Protein, HNF2 Protein, HPC11 Protein, LF-B3 Protein, LFB3 Protein, MODY5 Protein, TCF-2 Protein, TCF2 Protein, VHNF1 Protein, HNF-1b Protein, HNF1B Protein, AI385728 Protein, AI987804 Protein, HNF-1Beta Protein, Hnf1beta Protein, Tcf-2 Protein, Tcf2 Protein, vHNF1 Protein, vHNF-1 Protein, chunp6877 Protein, hnf1b Protein, mst Protein, tcf2 Protein, vhnf1 Protein, lfb3 Protein, hnf-1beta Protein, hnf1-beta Protein, hnf1beta Protein, HNF1g Protein, wu:fc23f06 Protein, HNF1 homeobox B Protein, HNF1 homeobox Ba Protein, HNF1 homeobox B S homeolog Protein, HNF1 homeobox B L homeolog Protein, HNF1 homeobox Bb Protein, HNF1B Protein, Hnf1b Protein, hnf1ba Protein, hnf1b.S Protein, hnf1b.L Protein, hnf1bb Protein
- 背景
- Hepatocyte nuclear factor 1-beta (HNF-1-beta) (HNF-1B) (Homeoprotein LFB3) (Transcription factor 2) (TCF-2) (Variant hepatic nuclear factor 1) (vHNF1),FUNCTION: Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:7900999, PubMed:17924661). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed:24204001). {ECO:0000250|UniProtKB:Q03365, ECO:0000269|PubMed:17924661, ECO:0000269|PubMed:24204001, ECO:0000269|PubMed:7900999}.
- 分子量
- 61.3 kDa
- UniProt
- P35680
- 途径
- Hormone Transport, Stem Cell Maintenance, Tube Formation
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