HAT1 Protein (AA 2-419) (His tag)
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- 抗原 See all HAT1 蛋白
- HAT1 (Histone Acetyltransferase 1 (HAT1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-419
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宿主
- 人
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资源
- Insect Cells
- 标记
- This HAT1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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AGFGAMEKFL VEYKSAVEKK LAEYKCNTNT AIELKLVRFP EDLENDIRTF FPEYTHQLFG DDETAFGYKG LKILLYYIAG SLSTMFRVEY ASKVDENFDC VEADDVEGKI RQIIPPGFCT NTNDFLSLLE KEVDFKPFGT LLHTYSVLSP TGGENFTFQI YKADMTCRGF REYHERLQTF LMWFIETASF IDVDDERWHY FLVFEKYNKD GATLFATVGY MTVYNYYVYP DKTRPRVSQM LILTPFQGQG HGAQLLETVH RYYTEFPTVL DITAEDPSKS YVKLRDFVLV KLCQDLPCFS REKLMQGFNE DMAIEAQQKF KINKQHARRV YEILRLLVTD MSDAEQYRSY RLDIKRRLIS PYKKKQRDLA KMRKCLRPEE LTNQMNQIEI SMQHEQLEES FQELVEDYRR VIERLAQE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human HAT1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product HAT1 蛋白
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Histone Acetyltransferase 1 (HAT1) (AA 1-334) protein (GST tag)
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Histone Acetyltransferase 1 (HAT1) (AA 310-419) protein (GST tag)HAT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Histone Acetyltransferase 1 (HAT1) (AA 20-341) protein (His tag)HAT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS-PAGE SDS
Histone Acetyltransferase 1 (HAT1) proteinHAT1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HAT1 (Histone Acetyltransferase 1 (HAT1))
- 别名
- HAT1 (HAT1 产品)
- 别名
- KAT1 Protein, HAC07 Protein, HAC7 Protein, HAG02 Protein, MIK19.19 Protein, MIK19_19 Protein, histone acetyltransferase of the GNAT family 2 Protein, hac106 Protein, hat1 Protein, zgc:92639 Protein, histone acetyltransferase 1 Protein, histone acetyltransferase of the GNAT family 2 Protein, histone acetyltransferase catalytic subunit Protein, histone acetyltransferase Hat1 Protein, histone acetyltransferase 1 L homeolog Protein, HAT1 Protein, Hat1 Protein, HAG2 Protein, hat1 Protein, hat1.L Protein
- 背景
- Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. {ECO:0000269|PubMed:11585814, ECO:0000269|PubMed:20953179, ECO:0000269|PubMed:22615379, ECO:0000269|PubMed:9427644}.
- 分子量
- 50.3 kDa Including tag.
- UniProt
- O14929
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