ERLIN2 Protein (AA 25-339) (His tag)
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- 抗原 See all ERLIN2 蛋白
- ERLIN2 (ER Lipid Raft Associated 2 (ERLIN2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-339
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ERLIN2 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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KIEEGHIGVY YRGGALLTST SGPGFHLMLP FITSYKSVQT TLQTDEVKNV PCGTSGGVMI YFDRIEVVNF LVPNAVYDIV KNYTADYDKA LIFNKIHHEL NQFCSVHTLQ EVYIELFDQI DENLKLALQQ DLTSMAPGLV IQAVRVTKPN IPEAIRRNYE LMESEKTKLL IAAQKQKVVE KEAETERKKA LIEAEKVAQV AEITYGQKVM EKETEKKISE IEDAAFLARE KAKADAECYT AMKIAEANKL KLTPEYLQLM KYKAIASNSK IYFGKDIPNM FMDSAGSVSK QFEGLADKLS FGLEDEPLET ATKEN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ERLIN2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ERLIN2 蛋白
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ER Lipid Raft Associated 2 (ERLIN2) (AA 1-339) protein (GST tag)
ERLIN2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
ER Lipid Raft Associated 2 (ERLIN2) (AA 1-339) protein (Strep Tag)Crystallography grade ERLIN2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
ER Lipid Raft Associated 2 (ERLIN2) (AA 1-340) protein (Strep Tag)Crystallography grade ERLIN2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
ER Lipid Raft Associated 2 (ERLIN2) (AA 25-340) protein (His tag)Crystallography grade ERLIN2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ERLIN2 (ER Lipid Raft Associated 2 (ERLIN2))
- 别名
- ERLIN2 (ERLIN2 产品)
- 别名
- C8orf2 Protein, Erlin-2 Protein, NET32 Protein, SPFH2 Protein, SPG18 Protein, BC036333 Protein, C87251 Protein, Spfh2 Protein, Erlin-2-B Protein, erlin1 Protein, spfh1 Protein, spfh2 Protein, spfh2-B Protein, RGD1309010 Protein, SPFH domain-containing protein 2-A Protein, erlin2 Protein, erlin2-a Protein, erlin2-b Protein, spfh2-A Protein, ER lipid raft associated 2 Protein, erlin-2 Protein, Erlin-2 Protein, ER lipid raft associated 2 S homeolog Protein, ER lipid raft associated 2 L homeolog Protein, ERLIN2 Protein, erlin2 Protein, Tsp_03442 Protein, erln2 Protein, Erlin2 Protein, erlin2.S Protein, erlin2.L Protein
- 背景
- Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed:19240031, PubMed:17502376). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed:21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed:24217618). {ECO:0000269|PubMed:17502376, ECO:0000269|PubMed:19240031, ECO:0000269|PubMed:21343306, ECO:0000269|PubMed:24217618}.
- 分子量
- 36.4 kDa Including tag.
- UniProt
- O94905
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