Desmocollin 3 Protein (DSC3) (AA 712-896) (His tag)
(1 reference)-
- 抗原 See all Desmocollin 3 (DSC3) 蛋白
- Desmocollin 3 (DSC3)
- 蛋白类型
- Recombinant
- 产品特性
- AA 712-896
-
宿主
- 人
-
资源
- Insect Cells
- 标记
- This Desmocollin 3 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
-
MCGVFGATKG KRFPEDLAQQ NLIISNTEAP GDDRVCSANG FMTQTTNNSS QGFCGTMGSG M KNGGQETIEM MKGGNQTLES CRGAGHHHTL DSCRGGHTEV DNCRYTYSEW HSFTQPRLGE KLHRCNQNED RMPSQDYVLT YNYEGRGSPA GSVGCCSEKQ EEDGLDFLNN LEPKFITLAE ACTKRHHHHH H
Sequence includes C-terminal His-tag - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
- The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
-
Desmocollin 3 (DSC3) (AA 585-684) protein (GST tag)
DSC3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Desmocollin 3 (DSC3) (AA 717-896) protein (His tag)Crystallography grade DSC3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Desmocollin 3 (DSC3) (AA 136-690) protein (His tag)Crystallography grade DSC3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Desmocollin 3 (DSC3) (AA 136-695) protein (His tag)Crystallography grade DSC3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Desmocollin 3 (DSC3) (AA 136-896) protein (rho-1D4 tag)Crystallography grade DSC3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Desmocollin 3 (DSC3) (AA 136-896) protein (rho-1D4 tag)Crystallography grade DSC3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- In solution (20 mM Hepes, pH 7.4, 100 mM NaCl,)
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
-
: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).
-
: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).
-
- 抗原
- Desmocollin 3 (DSC3)
- 别名
- DSC3 (DSC3 产品)
- 别名
- CDHF3 Protein, DSC Protein, DSC1 Protein, DSC2 Protein, DSC4 Protein, HT-CP Protein, 5430426I24Rik Protein, MGC85083 Protein, desmocollin 3 Protein, desmocollin 3 S homeolog Protein, DSC3 Protein, Dsc3 Protein, dsc3.S Protein, dsc3 Protein
- 背景
- Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.
- 分子量
- 21.3 kDa Including tag
- UniProt
- Q14574
-
