Aspartyl Aminopeptidase Protein (DNPEP) (AA 1-475) (His tag)
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- 抗原 See all Aspartyl Aminopeptidase (DNPEP) 蛋白
- Aspartyl Aminopeptidase (DNPEP)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-475
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宿主
- 人
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资源
- Insect Cells
- 标记
- This Aspartyl Aminopeptidase protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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MQVAMNGKAR KEAVQTAAKE LLKFVNRSPS PFHAVAECRN RLLQAGFSEL KETEKWNIKP ESKYFMTRNS STIIAFAVGG QYVPGNGFSL IGAHTDSPCL RVKRRSRRSQ VGFQQVGVET YGGGIWSTWF DRDLTLAGRV IVKCPTSGRL EQQLVHVERP ILRIPHLAIH LQRNINENFG PNTEMHLVPI LATAIQEELE KGTPEPGPLN AVDERHHSVL MSLLCAHLGL SPKDIVEMEL CLADTQPAVL GGAYDEFIFA PRLDNLHSCF CALQALIDSC AGPGSLATEP HVRMVTLYDN EEVGSESAQG AQSLLTELVL RRISASCQHP TAFEEAIPKS FMISADMAHA VHPNYLDKHE ENHRPLFHKG PVIKVNSKQR YASNAVSEAL IREVANKVKV PLQDLMVRND TPCGTTIGPI LASRLGLRVL DLGSPQLAMH SIREMACTTG VLQTLTLFKG FFELFPSLSH NLLVD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human DNPEP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product DNPEP 蛋白
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Aspartyl Aminopeptidase (DNPEP) (AA 1-219) protein (His-IF2DI Tag)
DNPEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Aspartyl Aminopeptidase (DNPEP) (AA 1-475) protein (GST tag)DNPEP 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Aspartyl Aminopeptidase (DNPEP) (AA 1-473) protein (Strep Tag)Crystallography grade DNPEP 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Aspartyl Aminopeptidase (DNPEP) (AA 1-475), (full length) protein (His-SUMO Tag)DNPEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Aspartyl Aminopeptidase (DNPEP) protein (Myc-DYKDDDDK Tag)DNPEP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Aspartyl Aminopeptidase (DNPEP)
- 别名
- DNPEP (DNPEP 产品)
- 别名
- zgc:55944 Protein, MGC80319 Protein, PSPTO3876 Protein, DDBDRAFT_0186837 Protein, DDBDRAFT_0266791 Protein, DDB_0186837 Protein, DDB_0266791 Protein, dnpep Protein, ASPEP Protein, DAP Protein, AA407814 Protein, aspartyl aminopeptidase Protein, aspartyl aminopeptidase L homeolog Protein, Aspartyl aminopeptidase Protein, dnpep Protein, DNPEP Protein, dnpep.L Protein, PSPTO_3876 Protein, DNP1 Protein, LbPR_M2 Protein, Arch_1448 Protein, Saut_1010 Protein, Ilyop_2166 Protein, Sulku_0925 Protein, TSTA_003400 Protein, Intca_2758 Protein, Despr_2295 Protein, Odosp_2771 Protein, Selsp_1665 Protein, Dnpep Protein
- 背景
- Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism. {ECO:0000269|PubMed:9632644}.
- 分子量
- 53.4 kDa Including tag.
- UniProt
- Q9ULA0
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