DCMP Deaminase (DCTD) (AA 1-178) protein (Strep Tag)
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- 抗原 See all DCMP Deaminase (DCTD) 蛋白
- DCMP Deaminase (DCTD)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-178
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- Strep Tag
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product DCTD 蛋白
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DCMP Deaminase (DCTD) (AA 1-178) protein (His tag)
DCTD 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
DCMP Deaminase (DCTD) (AA 1-189) protein (GST tag)DCTD 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
DCMP Deaminase (DCTD) (AA 69-178) protein (GST tag)DCTD 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
DCMP Deaminase (DCTD) (AA 1-178) protein (Strep Tag)Crystallography grade DCTD 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
DCMP Deaminase (DCTD) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DCTD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
DCMP Deaminase (DCTD) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DCTD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- DCMP Deaminase (DCTD)
- 别名
- DCTD (DCTD 产品)
- 别名
- zgc:110226 Protein, DCTD Protein, 6030466N05Rik Protein, Afu2g06240 Protein, MGC81193 Protein, dCMP deaminase Protein, deoxycytidylate deaminase Protein, cell division protein DedD Protein, dCMP deaminase L homeolog Protein, conserved uncharacterised protein Protein, DCTD Protein, dctd Protein, dctD Protein, Dctd Protein, AFUA_2G06240 Protein, ACLA_068070 Protein, LELG_03636 Protein, VIBHAR_RS20285 Protein, LOC5564022 Protein, Ccur_00700 Protein, PAAG_00286 Protein, SPAP_0719 Protein, LOC100285892 Protein, dctd.L Protein, CNB00950 Protein, THAPS_42740 Protein, dCMP deaminase Protein, Thena_0587 Protein, Ccan_15560 Protein
- 物质类
- Viral Protein
- 背景
- Deoxycytidylate deaminase (EC 3.5.4.12) (dCMP deaminase),FUNCTION: Catalyzes the deamination of dCMP to dUMP, providing the nucleoside monophosphate substrate for the thymidylate synthase/TYMS (PubMed:7685356). Also, part of a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) in a two-step process entailing deamination to cytotoxic 5-hydroxymethyl-dUMP (hmdUMP), followed by its hydrolysis into 5-hydroxymethyluracil (hmU) and 2-deoxy-D-ribose 5-phosphate (deoxyribosephosphate) (PubMed:33833118). Catalyzes the first step in that pathway, the deamination of 5-hydroxymethyl-dCMP (hmdCMP) (PubMed:33833118). {ECO:0000269|PubMed:33833118, ECO:0000269|PubMed:7685356}.
- 分子量
- 20.0 kDa
- UniProt
- P32321
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