SIAE Protein (AA 24-523) (His tag)
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- 抗原 See all SIAE 蛋白
- SIAE (Sialic Acid Acetylesterase (SIAE))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-523
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宿主
- 人
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资源
- Insect Cells
- 标记
- This SIAE protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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IGFRFASYIN NDMVLQKEPA GAVIWGFGTP GATVTVTLRQ GQETIMKKVT SVKAHSDTWM VVLDPMKPGG PFEVMAQQTL EKINFTLRVH DVLFGDVWLC SGQSNMQMTV LQIFNATREL SNTAAYQSVR ILSVSPIQAE QELEDLVAVD LQWSKPTSEN LGHGYFKYMS AVCWLFGRHL YDTLQYPIGL IASSWGGTPI EAWSSGRSLK ACGVPKQGSI PYDSVTGPSK HSVLWNAMIH PLCNMTLKGV VWYQGESNIN YNTDLYNCTF PALIEDWRET FHRGSQGQTE RFFPFGLVQL SSDLSKKSSD DGFPQIRWHQ TADFGYVPNP KMPNTFMAVA MDLCDRDSPF GSIHPRDKQT VAYRLHLGAR ALAYGEKNLT FEGPLPEKIE LLAHKGLLNL TYYQQIQVQK KDNKIFEISC CSDHRCKWLP ASMNTVSTQS LTLAIDSCHG TVVALRYAWT TWPCEYKQCP LYHPSSALPA PPFIAFITDQ GPGHQSNVAK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SIAE Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SIAE 蛋白
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Sialic Acid Acetylesterase (SIAE) (AA 1-523) protein (GST tag)
SIAE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sialic Acid Acetylesterase (SIAE) (AA 24-275) protein (His tag)Crystallography grade SIAE 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sialic Acid Acetylesterase (SIAE) (AA 276-541) protein (His tag)Crystallography grade SIAE 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sialic Acid Acetylesterase (SIAE) protein (Myc-DYKDDDDK Tag)SIAE 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SIAE (Sialic Acid Acetylesterase (SIAE))
- 别名
- SIAE (SIAE 产品)
- 别名
- MGC68688 Protein, MGC137441 Protein, zgc:56454 Protein, YSG2 Protein, DKFZp469J191 Protein, LSE Protein, Ysg2 Protein, RGD1310431 Protein, AIS6 Protein, CSE-C Protein, CSEC Protein, sialic acid acetylesterase L homeolog Protein, sialic acid acetylesterase Protein, siae.L Protein, SIAE Protein, siae Protein, Siae Protein
- 背景
- Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.
- 分子量
- 56.9 kDa Including tag.
- UniProt
- Q9HAT2
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