VEGF Protein (AA 27-147) (AVI tag,His tag,Biotin)
(1 reference)-
- 抗原 See all VEGF 蛋白
- VEGF (Vascular Endothelial Growth Factor (VEGF))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 27-147
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This VEGF protein is labelled with AVI tag,His tag,Biotin.
- 品牌
- MABSol®,PrecisionAvi
- 序列
- AA 27-147
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 16.7 kDa. As a result of glycosylation, the protein migrates as 18 kDa and 22 kDa on a SDS-PAGE gel under reducing (R) condition and 35-40 kDa under non-reducing (NR) condition.
- 纯度
- >95 % as determined by reduced SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product VEGF 蛋白
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Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein
HPLC verified VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein (Biotin)VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (Active) proteinVEGF 宿主: 人 宿主: Yeast (Pichia pastoris) > 95 % by SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein (His tag)VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-190) (Active) proteinHPLC verified VEGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (Active) proteinVEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Vascular Endothelial Growth Factor (VEGF) (AA 205-324) protein (His tag)VEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Vascular Endothelial Growth Factor (VEGF) (AA 27-147) (Active) protein (His tag)VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-146) (Active) proteinVEGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
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: "Biophysical Characterization Platform Informs Protein Scaffold Evolvability." in: ACS combinatorial science, Vol. 21, Issue 4, pp. 323-335, (2019) (PubMed).
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: "Biophysical Characterization Platform Informs Protein Scaffold Evolvability." in: ACS combinatorial science, Vol. 21, Issue 4, pp. 323-335, (2019) (PubMed).
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- 抗原
- VEGF (Vascular Endothelial Growth Factor (VEGF))
- 别名
- VEGF (VEGF 产品)
- 别名
- MVCD1 Protein, VEGF Protein, VPF Protein, Vegf Protein, Vegf120 Protein, Vegf164 Protein, Vegf188 Protein, Vpf Protein, VEGF-A Protein, VEGF164 Protein, eVEGF120 Protein, eVEGF164 Protein, vegf-a Protein, vegf Protein, vascular endothelial growth factor A Protein, vascular endothelial growth factor A L homeolog Protein, vascular endothelial growth factor Protein, vascular endothelial growth factor precursor Protein, VEGFA Protein, Vegfa Protein, vegfa.L Protein, VEGF Protein, vegf Protein
- 背景
- Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87 % aa sequence identity with corresponding regions of mouse and rat, 93 % with feline, equine and bovine, and 91 %, 95 % and 96 % with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.
- 分子量
- 16.7 kDa
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