FcRn Protein (AA 24-297) (His tag,Strep Tag,AVI tag,Biotin)
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- 抗原 See all FcRn 蛋白
- FcRn (IgG receptor FcRn (FcRn))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 24-297
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This FcRn protein is labelled with His tag,Strep Tag,AVI tag,Biotin.
- 品牌
- MABSol®,PrecisionAvi
- 序列
- AA 24-297
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- The subunit FCGRT carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Strep II tag at the C-terminus. The protein has a calculated MW of 33 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 36 kDa (FCGRT), 14 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product FcRn 蛋白
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- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
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Phagocytosis by an HIV antibody is associated with reduced viremia irrespective of enhanced complement lysis." in: Nature communications, Vol. 13, Issue 1, pp. 662, (2022) (PubMed).
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Phagocytosis by an HIV antibody is associated with reduced viremia irrespective of enhanced complement lysis." in: Nature communications, Vol. 13, Issue 1, pp. 662, (2022) (PubMed).
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- 抗原
- FcRn (IgG receptor FcRn (FcRn))
- 别名
- FcRn (FcRn 产品)
- 别名
- FCRN Protein, alpha-chain Protein, FcRn Protein, Fc fragment of IgG receptor and transporter Protein, Fc receptor, IgG, alpha chain transporter Protein, FCGRT Protein, Fcgrt Protein
- 背景
- FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
- 分子量
- 34.1 kDa (FCGRT) & 13.1 kDa (B2M)
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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