NADK Protein (His tag)
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- 抗原 See all NADK 蛋白
- NADK (NAD Kinase (NADK))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- Mycobacterium tuberculosis
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资源
- 大肠杆菌（E. Coli）
- 标记
- This NADK protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 产品特性
- NAD kinase (Mycobacterium tuberculosis) is fused at the N-terminus to a His-tag.
- 纯度
- >98 % (SDS-PAGE)
- Top Product
- Discover our top product NADK 蛋白
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NAD Kinase (NADK) (AA 64-298) protein (His tag)
NADK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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~1.2 U/mg protein. (Substrate: NAD (+ ATP)) ~3.8 U/mg protein. (Substrate: NAD (+ poly(P)) One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. In 10 mM TRIS at pH 8.0, containing 1 mM MgCl2, 0.5 mM EDTA, 1 mM DTT and 1 mM PMSF.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C. - 有效期
- 6 months
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- 抗原
- NADK (NAD Kinase (NADK))
- 别名
- NAD Kinase (NADK 产品)
- 别名
- 4432404C02Rik Protein, BC004012 Protein, dJ283E3.1 Protein, NAD kinase Protein, Nadk Protein, NADK Protein
- 背景
- NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+
- 分子量
- ~33.0kDa
- UniProt
- P0A5S6
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